Mutation of glucose-methanol-choline oxidoreductase leads to thermosensitive genic male sterility in rice and Arabidopsis.

Content Provider	Europe PMC
Author	Zhang, Yan‐Fei Li, Yue‐Ling Zhong, Xiang Wang, Jun‐Jie Zhou, Lei Han, Yu Li, Dan‐Dan Wang, Na Huang, Xue‐Hui Zhu, Jun Yang, Zhong‐Nan
Abstract	SummaryThermosensitive genic male sterility (TGMS) lines serve as the major genetic resource for two‐line hybrid breeding in rice. However, their unstable sterility under occasional low temperatures in summer highly limits their application. In this study, we identified a novel rice TGMS line, ostms18, of cultivar ZH11 (Oryza sativa ssp. japonica). ostms18 sterility is more stable in summer than the TGMS line carrying the widely used locus tms5 in the ZH11 genetic background, suggesting its potential application for rice breeding. The ostms18 TGMS trait is caused by the point mutation from Gly to Ser in a glucose‐methanol‐choline (GMC) oxidoreductase; knockout of the oxidoreductase was previously reported to cause complete male sterility. Cellular analysis revealed the pollen wall of ostms18 to be defective, leading to aborted pollen under high temperature. Further analysis showed that the tapetal transcription factor OsMS188 directly regulates OsTMS18 for pollen wall formation. Under low temperature, the flawed pollen wall in ostms18 is sufficient to protect its microspore, allowing for development of functional pollen and restoring fertility. We identified the orthologous gene in Arabidopsis. Although mutants for the gene were fertile under normal conditions (24°C), fertility was significantly reduced under high temperature (28°C), exhibiting a TGMS trait. A cellular mechanism integrated with genetic mutations and different plant species for fertility restoration of TGMS lines is proposed.
Page Count	2035
ISSN	14677644
Journal	Plant Biotechnology Journal
Volume Number	20
PubMed Central reference number	PMC9491461
Issue Number	10
PubMed reference number	35781755
e-ISSN	14677652
DOI	10.1111/pbi.13886
Language	English
Publisher	John Wiley and Sons Inc.
Publisher Date	2022-07-26
Publisher Place	Hoboken
Access Restriction	Open
Rights License	This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. © 2022 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
Subject Keyword	rice pollen wall GMC oxidoreductase thermosensitive genic male sterility
Content Type	Text
Resource Type	Article
Subject	Plant Science Biotechnology Agronomy and Crop Science