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Synthesis of Cu-doped carbon dot/chitosan film composite as a catalyst for the colorimetric detection of hydrogen peroxide and glucose.
| Content Provider | Europe PMC |
|---|---|
| Author | Tummala, Srikrishna Bandi, Rajkumar Ho, Yen-Peng |
| Abstract | The use of colloidal nanoparticles suffers from the drawbacks of potential color interference and substrate-induced aggregation. To overcome the limitations, a catalyst was developed by crosslinking Cu-doped carbon dots (Cu-CDs) with chitosan. Cu-CDs with high peroxidase activity were prepared by using a rapid microwave-assisted method. The Cu-CDs containing 6.88% of Cu had an average particle size of 2.25 nm and exhibited 9% of fluorescence quantum yield. The nanozyme/film composite was prepared by crosslinking between the amino groups of Cu-CDs and those of chitosan via a glutaraldehyde linker. A H2O2-mediated tetramethylbenzidine (TMB) oxidation reaction was use to evaluate the peroxidase activity of the film. Based on the TMB color changes, colorimetric assays were developed for the detection of H2O2 and glucose at an absorption wavelength 652 nm. Under the optimal conditions, the linear ranges for H2O2 and glucose were 0.625–40 µM and 1.9–125 µM, respectively, and the detection limits were 0.12 µM and 0.69 µM, respectively. The colorimetric assay was also applied to analyze diluted human serum samples spiked with glucose. Furthermore, this biodegradable, non-toxic, and easy-to-handle nanozyme composite could be stored for over 4 weeks without a significant decrease in activity.Graphical abstractSupplementary InformationThe online version contains supplementary material available at 10.1007/s00604-022-05386-3. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC9293876&blobtype=pdf |
| ISSN | 00263672 |
| Journal | Mikrochimica Acta [Mikrochim Acta] |
| Volume Number | 189 |
| DOI | 10.1007/s00604-022-05386-3 |
| PubMed Central reference number | PMC9293876 |
| Issue Number | 8 |
| PubMed reference number | 35851663 |
| e-ISSN | 14365073 |
| Language | English |
| Publisher | Springer Vienna |
| Publisher Date | 2022-07-19 |
| Publisher Place | Vienna |
| Access Restriction | Open |
| Rights License | Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2022 |
| Subject Keyword | Cu-carbon dots/chitosan film Enzyme Peroxidase mimic Hydrogen peroxide Glucose Glucose oxidase |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry |
