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Self-Assembled Nanobodies as Selectively Targeted, Nanostructured, and Multivalent Materials.
| Content Provider | Europe PMC |
|---|---|
| Author | Sánchez-García, Laura Voltà-Durán, Eric Parladé, Eloi Mazzega, Elisa Sánchez-Chardi, Alejandro Serna, Naroa López-Laguna, Hèctor Mitstorfer, Mara Unzueta, Ugutz Vázquez, Esther Villaverde, Antonio de Marco, Ario |
| Copyright Year | 2021 |
| Abstract | Nanobodies represent valuable toolsin advanced therapeutic strategiesbut their small size (∼2.5 × ∼ 4 nm) and limitedvalence for interactions might pose restrictions for in vivo applications, especially regarding their modest capacity for multivalentand cooperative interaction. In this work, modular protein constructshave been designed, in which nanobodies are fused to protein domainsto provide further functionalities and to favor oligomerization intostable self-assembled nanoparticles. The nanobody specificity fortheir targets is maintained in such supramolecular complexes. Also,their diameter around 70 nm and multivalent interactivity should favorbinding and penetrability into target cells via solvent-exposed receptor.These concepts have been supported by unrelated nanobodies directedagainst the ricin toxin (A3C8) and the Her2 receptor (EM1), respectively,that were modified with the addition of a reporter protein and a hexa-histidinetag at the C-terminus that promotes self-assembling. The A3C8-basednanoparticles neutralize the ricin toxin efficiently, whereas theEM1-based nanoparticles enable to selective imaging Her2-positivecells. These findings support the excellent extracellular and intracellularfunctionality of nanobodies organized in form of oligomeric nanoscaleassemblies. |
| ISSN | 19448244 |
| Journal | ACS Applied Materials & Interfaces |
| Volume Number | 13 |
| PubMed Central reference number | PMC9262252 |
| Issue Number | 25 |
| PubMed reference number | 34129336 |
| e-ISSN | 19448252 |
| DOI | 10.1021/acsami.1c08092 |
| Language | English |
| Publisher | AmericanChemical Society |
| Publisher Date | 2021-06-15 |
| Access Restriction | Open |
| Rights License | Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). © 2021 American Chemical Society |
| Subject Keyword | nanobodies self-assembling ricin nanoparticles controlled delivery biomaterials |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Materials Science |
