Detection of Human Papillomavirus and p16^INK4a Expression in Thai Patients with Oral Squamous Cell Carcinoma.

Content Provider	Europe PMC
Author	Rungraungrayabkul, Dulyapong Panpradit, Naruemon Lapthanasupkul, Puangwan Kitkumthorn, Nakarin Klanrit, Poramaporn Subarnbhesaj, Ajiravudh Sresumatchai, Vanvisa Klongnoi, Boworn Khovidhunkit, Siribang-on Piboonniyom
Abstract	This study investigated the prevalence of human papillomavirus (HPV) infection in oral squamous cell carcinoma (OSCC) cases, as well as the association between HPV presence and p16INK4a expression, in Thai patients with OSCC. Eighty-one formalin-fixed paraffin-embedded specimens of OSCC were obtained. DNA extraction was performed; this was followed by nested polymerase chain reaction analysis to determine HPV DNA status, using consensus primers for the L1 region of HPV. HPV subtypes were determined by DNA sequencing. HPV-positive specimens and HPV-negative specimens from age- and sex-matched patients were subjected to immunohistochemical analysis to determine p16INK4a expression status. Of the 81 OSCC specimens, eight (9.9%) exhibited HPV DNA; DNA sequencing confirmed that the viral subtype was HPV-18 in all eight specimens. These eight HPV-positive specimens, as well as eight HPV-negative specimens from age- and sex-matched patients, were subjected to immunohistochemical analysis to determine p16INK4a expression status. Three of eight (37.8%) HPV-positive specimens and three of eight (37.8%) HPV-negative specimens showed positive p16INK4a expression findings. However, we did not find a significant association between HPV status and p16INK4a expression status in our OSCC samples. In conclusion, the prevalence of high-risk HPV was low in this group of OSCC patients; no association between HPV status and p16INK4a expression status was identified.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC9187793&blobtype=pdf
ISSN	1936055X
Volume Number	16
DOI	10.1007/s12105-021-01381-x
PubMed Central reference number	PMC9187793
Issue Number	2
PubMed reference number	34590266
Journal	Head and Neck Pathology [Head Neck Pathol]
e-ISSN	19360568
Language	English
Publisher	Springer US
Publisher Date	2021-09-29
Publisher Place	New York
Access Restriction	Open
Rights License	© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021
Subject Keyword	HPV Oral cancer OSCC PCR p16INK4a Thai
Content Type	Text
Resource Type	Article
Subject	Otorhinolaryngology Pathology and Forensic Medicine Oncology