Comparative evaluation of microRNA-155 expression level and its correlation with tumor necrotizing factor α and interleukin 6 in patients with chronic periodontitis.

Content Provider	Europe PMC
Author	Mogharehabed, Ahmad Yaghini, Jaber Aminzadeh, Ania Rahaiee, Mahdi
Copyright Year	2022
Abstract	Background:MicroRNAs are a class of small noncoding ribonucleic acids that perform a critical role in adjustment of gene expression. miRNAs-155 (miR-155) participates in controlling inflammation. Periodontitis is defined as inflammatory disorder of tissues surrounding the teeth. In this study, the expression levels of miR-155 and its target genes, tumor necrotizing factor alpha (TNF-α), and interleukin-6 (IL-6) were evaluated in a group of Iranian patients.Materials and Methods:This sectional study was performed on 10 healthy controls and 10 individuals with chronic periodontitis by means of polymerase chain reaction (PCR) test. For each individual, clinical parameters including probing depth and clinical attachment loss and blood samples were measured. Levels of miR-155, TNF-α, and IL-6 were quantified using real-time PCR (α=0/05) and the results were analyzed by Mann–Whitney U test.Results:The level of miR-155 was significantly higher in patients with chronic periodontitis (P < 0.001). A positive correlation was observed between the level of miR-155 and clinical parameters (P < 0.05). Level of miR-155 in tissue samples was correlated with blood samples although the expression level was higher in blood samples.Conclusion:As the expression level of miR-155, TNF-α, and IL-6 genes was higher in subjects with chronic periodontitis than healthy individuals, it might suggest a role for miR-155 in patients with chronic periodontitis.
ISSN	17353327
Journal	Dental Research Journal
Volume Number	19
PubMed Central reference number	PMC9164665
PubMed reference number	35669607
e-ISSN	20080255
Language	English
Publisher	Wolters Kluwer - Medknow
Publisher Date	2022-04-27
Publisher Place	India
Access Restriction	Open
Rights License	This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. Copyright: © 2022 Dental Research Journal
Subject Keyword	Interleukin-6 microRNAs microRNA-155 periodontitis tumor necrosis factor-alpha
Content Type	Text
Resource Type	Article
Subject	Dentistry