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XNAs: A Troubleshooter for Nucleic Acid Sensing.
| Content Provider | Europe PMC |
|---|---|
| Author | Mana, Tanushree Bhattacharya, Budhaditya Lahiri, Hiya Mukhopadhyay, Rupa |
| Copyright Year | 2022 |
| Abstract | Thestrategies fornucleic acid sensing based on nucleic acid hybridizationbetween the target sequence and the capture probe sequence are consideredto be largely successful as far as detection of a specific targetof known sequence is concerned. However, when compared with othercomplementary methods, like direct sequencing, a number of resultsare still found to be either “false positives” or “falsenegatives”. This suggests that modifications in these strategiesare necessary to make them more accurate. In this minireview, we proposethat one way toward improvement could be replacement of the DNA captureprobes with the xeno nucleic acid or XNA capture probes. This is becausethe XNAs, especially the locked nucleic acid, the peptide nucleicacid, and the morpholino, have shown better single nucleobase mismatchdiscrimination capacity than the DNA capture probes, indicating theircapacity for more precise detection of nucleic acid sequences, whichis beneficial for detection of gene stretches having point mutations.Keeping the current trend in mind, this minireview will include therecent developments in nanoscale, fluorescent label-free applications,and present the cases where the XNA probes show clear advantages overthe DNA probes. |
| Journal | ACS Omega |
| Volume Number | 7 |
| PubMed Central reference number | PMC9096816 |
| Issue Number | 18 |
| PubMed reference number | 35571783 |
| e-ISSN | 24701343 |
| DOI | 10.1021/acsomega.2c00581 |
| Language | English |
| Publisher | American Chemical Society |
| Publisher Date | 2022-04-28 |
| Access Restriction | Open |
| Rights License | Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). © 2022 The Authors. Published by American Chemical Society |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Chemical Engineering |
