Enzymatic Effectiveness of Alcoholic and Aqueous Extract of Salvia Officinalis in Mice Poisoned with Tetrachloride.

Content Provider	Europe PMC
Author	Kadhim, R Ali, N. H Aziz Ibrahim, D
Description	Regarding the antioxidant, anti-inflammatory, and antibacterial effects of Salvia officnalis (S. officinalis) extracts and the use of medicinal herbs as an alternative to chemical drugs, this study aimed to evaluate the enzymatic changes and reduction of hepatocyte damage in mice poisoned with carbon tetrachloride (CCl4) after treatment with aqueous and alcoholic extract of Salvia officnalis. A total of 40 adult male mice were divided into eight groups including six experimental, one negative, and one positive control group, which were exposed to CCl4 at the concentration of 2.3 mg/kg. The active compounds in the alcoholic and aqueous extracts of S. officinalis were obtained using high-performance liquid chromatography. Subsequently, S. officinalis extract in 100, 200, and 300 mg /kg doses were fed orally to mice for six days. The enzymes (GST, ALP, ALT, AST, and MDA) were determined in mice serum. The study results showed that enzyme activity was significantly decreased in the group treated with S. officinalis extract, and the concentration of 300mg/kg proved to be most effective. In addition, it was indicated that the alcoholic extract had a higher effect than the aqueous extract, which might be due to the greater amount of active compounds in the alcoholic extract. The improving effects of S. officinalis can be attributed to the bioactive components with antioxidant properties that inhibit the damaging effects of free radicals, chemical drugs, and tissue damage.
Abstract	Regarding the antioxidant, anti-inflammatory, and antibacterial effects of Salvia officnalis (S. officinalis) extracts and the use of medicinal herbs as an alternative to chemical drugs,this study aimed to evaluate the enzymatic changes and reduction of hepatocyte damage in mice poisoned with carbon tetrachloride (CCl4) after treatment with aqueousand alcoholic extract of Salvia officnalis. A total of 40 adult male mice were divided into eight groups including six experimental, one negative, and one positive control group,which were exposed to CCl4 at the concentration of 2.3 mg/kg. The active compounds in the alcoholic and aqueous extracts of S. officinalis were obtained using high-performanceliquid chromatography. Subsequently, S. officinalis extract in 100, 200, and 300 mg /kg doses were fed orally to mice for six days. The enzymes (GST, ALP, ALT, AST, and MDA)were determined in mice serum. The study results showed that enzyme activity was significantly decreased in the group treated with S. officinalis extract, and theconcentration of 300mg/kg proved to be most effective. In addition, it was indicated that the alcoholic extract had a higher effect than the aqueous extract, which might be due tothe greater amount of active compounds in the alcoholic extract. The improving effects of S. officinalis can be attributed to the bioactive components with antioxidant propertiesthat inhibit the damaging effects of free radicals, chemical drugs, and tissue damage.
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ISSN	03653439
Volume Number	76
PubMed Central reference number	PMC9083868
Issue Number	6
PubMed reference number	35546970
Journal	Archives of Razi Institute [Arch Razi Inst]
e-ISSN	20089872
DOI	10.22092/ari.2021.356239.1812
Language	English
Publisher	Razi Vaccine & Serum Research Institute
Publisher Date	2021-12-30
Publisher Place	Iran
Access Restriction	Open
Subject Keyword	Alcoholic extract Aqueous extract CCl4 Enzymatic effective Salvia officnalis
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Veterinary Toxicology