NDLI: MiR-130a-3p regulates neural stem cell differentiation in vitro by targeting Acsl4.

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MiR-130a-3p regulates neural stem cell differentiation in vitro by targeting Acsl4.

Content Provider	Europe PMC
Author	Li, Wen Shan, Bo‐Quan Zhao, He‐Yan He, Hui Tian, Mei‐Ling Cheng, Xiang Qin, Jian‐Bing Jin, Guo‐Hua
Abstract	AbstractIn the adult mammalian brain, neural stem cells (NSCs) are the precursor cells of neurons that contribute to nervous system development, regeneration, and repair. MicroRNAs (miRNAs) are small non‐coding RNAs that regulate cell fate determination and differentiation by negatively regulating gene expression. Here, we identified a post‐transcriptional mechanism, centred around miR‐130a‐3p that regulated NSC differentiation. Importantly, overexpressing miR‐130a‐3p promoted NSC differentiation into neurons, whereas inhibiting miR‐130a‐3p function reduced the number of neurons. Then, the quantitative PCR, Western blot and dual‐luciferase reporter assays showed that miR‐130a‐3p negatively regulated acyl‐CoA synthetase long‐chain family member 4 (Acsl4) expression. Additionally, inhibition of Acsl4 promoted NSC differentiation into neurons, whereas silencing miR‐130a‐3p partially suppressed the neuronal differentiation induced by inhibiting Acsl4. Furthermore, overexpressing miR‐130a‐3p or inhibiting Acsl4 increased the levels of p‐AKT, p‐GSK‐3β and PI3K. In conclusion, our results suggested that miR‐130a‐3p targeted Acsl4 to promote neuronal differentiation of NSCs via regulating the Akt/PI3K pathway. These findings may help to develop strategies for stem cell‐mediated treatment for central nervous system diseases.
Page Count	11
ISSN	15821838
Journal	Journal of Cellular and Molecular Medicine
Volume Number	26
PubMed Central reference number	PMC9077303
Issue Number	9
PubMed reference number	35429110
e-ISSN	15824934
DOI	10.1111/jcmm.17285
Language	English
Publisher	John Wiley and Sons Inc.
Publisher Date	2022-04-16
Publisher Place	Hoboken
Access Restriction	Open
Rights License	This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2022 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
Subject Keyword	Acsl4 Akt/PI3K signalling pathway differentiation MiR‐130a‐3p neural stem cells
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Medicine

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