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Impact of Bicarbonate Interference on Routine Ion-Selective Electrode Chloride Measurements.
| Content Provider | Europe PMC |
|---|---|
| Author | Monteyne, Tinne Oyaert, Matthijs Van Dalem, Annelien Godefroid, Maaike Cuykx, Matthias Callewaert, Nico |
| Copyright Year | 2022 |
| Abstract | BackgroundChloride measurement is crucial for calculating the anion gap. Bicarbonate can interfere with chloride measurements; however, there is no information on the specific types of electrodes affected or the changes in bicarbonate non-selectivity over time. We evaluated the interference of bicarbonate on chloride measurements using different electrodes and the stability of this interference over time.MethodsThe effect of bicarbonate on chloride measured with electrodes of various manufacturers was assessed. When non-selectivity toward bicarbonate was observed, the stability of this interference during the electrode’s lifetime was explored. The impact of the bicarbonate concentration on the calibrator was also evaluated.ResultsNon-selectivity was observed for electrodes using quaternary ammonium salts (Beckman Coulter, Siemens, and Roche), with overestimated or underestimated chloride values observed at high or low bicarbonate concentrations, respectively. The degree of selectivity varied among electrodes. With the Roche electrode, interference became more pronounced over time, whereas the Siemens electrode appeared to gain selectivity during its lifetime. For the Roche system, adjusting the calibrator’s bicarbonate concentration from 30 mmol/L to 20–24 mmol/L reduced the number of samples with unacceptable bias (>3%) from 77.3% to 12.6%. Lot-to-lot variations in the calibrator bicarbonate concentration increased the uncertainty of chloride measurements.ConclusionsThe extent of bicarbonate-induced error varied according to the type, manufacturer, and wear of the electrode; the bicarbonate concentration in the calibrators and the tested sample; and the chloride content. Laboratories should be aware of the impact of bicarbonate on routine chloride measurements to establish appropriate QC procedures. |
| ISSN | 22343806 |
| Journal | Annals of Laboratory Medicine |
| Volume Number | 42 |
| PubMed Central reference number | PMC9057815 |
| Issue Number | 5 |
| PubMed reference number | 35470274 |
| e-ISSN | 22343814 |
| DOI | 10.3343/alm.2022.42.5.566 |
| Language | English |
| Publisher | Korean Society for Laboratory Medicine |
| Publisher Date | 2022-09-01 |
| Access Restriction | Open |
| Rights License | This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. © Korean Society for Laboratory Medicine |
| Subject Keyword | Chloride Bicarbonate Ion-selective electrode |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry (medical) Clinical Biochemistry |
