Cellular Interaction of Bone Marrow Mesenchymal Stem Cells with Polymer and Hydrogel 3D Microscaffold Templates.

Content Provider	Europe PMC
Author	Costa, BeatrizN. L. Adão, Ricardo M. R. Maibohm, Christian Accardo, Angelo Cardoso, Vanessa F. Nieder, Jana B.
Copyright Year	2022
Abstract	Biomimicking biologicalniches of healthy tissues or tumors canbe achieved by means of artificial microenvironments, where structuraland mechanical properties are crucial parameters to promote tissueformation and recreate natural conditions. In this work, three-dimensional(3D) scaffolds based on woodpile structures were fabricated by two-photonpolymerization (2PP) of different photosensitive polymers (IP-S andSZ2080) and hydrogels (PEGDA 700) using two different 2PP setups,a commercial one and a customized one. The structures’ propertieswere tuned to study the effect of scaffold dimensions (gap size) andtheir mechanical properties on the adhesion and proliferation of bonemarrow mesenchymal stem cells (BM-MSCs), which can serve as a modelfor leukemic diseases, among other hematological applications. Thewoodpile structures feature gap sizes of 25, 50, and 100 μmand a fixed beam diameter of 25 μm, to systematically studythe optimal cell colonization that promotes healthy cell growth andpotential tissue formation. The characterization of the scaffoldsinvolved scanning electron microscopy and mechanical nanoindenting,while their suitability for supporting cell growth was evaluated withlive/dead cell assays and multistaining 3D confocal imaging. In themechanical assays of the hydrogel material, we observed two differentstiffness ranges depending on the indentation depth. Larger gap woodpilestructures coated with fibronectin were identified as the most promisingscaffolds for 3D BM-MSC cellular models, showing higher proliferationrates. The results indicate that both the design and the employedmaterials are suitable for further assays, where retaining the BM-MSCstemness and original features is crucial, including studies focusedon BM disorders such as leukemia and others. Moreover, the combinationof 3D scaffold geometry and materials holds great potential for theinvestigation of cellular behaviors in a co-culture setting, for example,mesenchymal and hematopoietic stem cells, to be further applied inmedical research and pharmacological studies.
ISSN	19448244
Journal	ACS Applied Materials & Interfaces
Volume Number	14
PubMed Central reference number	PMC8949723
Issue Number	11
PubMed reference number	35282678
e-ISSN	19448252
DOI	10.1021/acsami.1c23442
Language	English
Publisher	American Chemical Society
Publisher Date	2022-03-13
Access Restriction	Open
Rights License	Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). © 2022 The Authors. Published by American Chemical Society
Subject Keyword	two-photon polymerization three-dimensional scaffolds woodpile structures polymer hydrogel bone marrow mesenchymalstem cells tissue engineering
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Materials Science