Redox imbalance induces remodeling of glucose metabolism in Rhipicephalus microplus embryonic cell line.

Content Provider	Europe PMC
Author	Della Noce, Bárbara Martins da Silva, Renato de Carvalho Uhl, Marcelle Vianna Konnai, Satoru Ohashi, Kazuhiko Calixto, Christiano Arcanjo, Angélica de Abreu, Leonardo Araujo de Carvalho, Stephanie Serafim da Silva Vaz, Itabajara Logullo, Carlos
Copyright Year	2022
Abstract	Carbohydrate metabolism not only functions in supplying cellular energy but also has an important role in maintaining physiological homeostasis and in preventing oxidative damage caused by reactive oxygen species. Previously, we showed that arthropod embryonic cell lines have high tolerance to H2O2 exposure. Here, we describe that Rhipicephalus microplus tick embryonic cell line (BME26) employs an adaptive glucose metabolism mechanism that confers tolerance to hydrogen peroxide at concentrations too high for other organisms. This adaptive mechanism sustained by glucose metabolism remodeling promotes cell survival and redox balance in BME26 cell line after millimolar H2O2 exposure. The present work shows that this tick cell line could tolerate high H2O2 concentrations by initiating a carbohydrate-related adaptive response. We demonstrate that gluconeogenesis was induced as a compensation strategy that involved, among other molecules, the metabolic enzymes NADP-ICDH, G6PDH, and PEPCK. We also found that this phenomenon was coupled to glycogen accumulation and glucose uptake, supporting the pentose phosphate pathway to sustain NADPH production and leading to cell survival and proliferation. Our findings suggest that the described response is not atypical, being also observed in cancer cells, which highlights the importance of this model to all proliferative cells. We propose that these results will be useful in generating basic biological information to support the development of new strategies for disease treatment and parasite control.
ISSN	00219258
Volume Number	298
PubMed Central reference number	PMC8857477
Issue Number	3
PubMed reference number	35063504
Journal	The Journal of Biological Chemistry [J. Biol. Chem]
e-ISSN	1083351X
DOI	10.1016/j.jbc.2022.101599
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2022-01-19
Access Restriction	Open
Rights License	This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). © 2022 The Authors
Subject Keyword	glucose metabolism embryogenesis ROS arthropod ETS, electron transport system G6P, glucose 6-phosphate G6PDH, glucose-6-phosphate dehydrogenase GDE, glycogen debranching enzyme GS, glycogen synthase GSK3β, glycogen synthase kinase 3 β HK, hexokinase ICDH, isocitrate dehydrogenase IGF, insulin-like growth factor ISP, insulin-signaling pathway LDH, lactate dehydrogenase OCR, oxygen consumption rate PEPCK, phosphoenolpyruvate carboxykinase PK, pyruvate kinase ROS, reactive oxygen species
Content Type	Text
Resource Type	Article
Subject	Cell Biology Molecular Biology Biochemistry