Local Connections of Pyramidal Neurons to Parvalbumin-Producing Interneurons in Motor-Associated Cortical Areas of Mice.

Content Provider	Europe PMC
Author	Kuramoto, Eriko Tanaka, Yasuhiro R. Hioki, Hiroyuki Goto, Tetsuya Kaneko, Takeshi
Copyright Year	2022
Abstract	Parvalbumin (PV)-producing neurons are the largest subpopulation of cortical GABAergic interneurons, which mediate lateral, feedforward, and feedback inhibition in local circuits and modulate the activity of pyramidal neurons. Clarifying the specific connectivity between pyramidal and PV neurons is essential for understanding the role of PV neurons in local circuits. In the present study, we visualized somas and dendrites of PV neurons using transgenic mice in which PV neurons specifically express membrane-targeted GFP, and intracellularly labeled local axons of 26 pyramidal neurons in layers 2–6 in acute slices of the motor-associated cortex from transgenic mice. We mapped morphologically distribution of inputs from a pyramidal neuron to PV neurons based on contact sites (appositions) between the axons from an intracellularly filled pyramidal neuron and the dendrites of PV neurons. Layer 6 corticothalamic (CT)-like pyramidal neurons formed appositions to PV neurons at a significantly higher rate than other pyramidal neurons. The percentage of apposed varicosities to all the labeled varicosities of layer 6 CT-like neurons was 28%, and that of the other pyramidal neurons was 12–19%. Layer 6 CT-like neurons preferentially formed appositions with PV neurons in layers 5b–6, while other pyramidal neurons uniformly formed appositions with PV neurons in all layers. Furthermore, both layer 6 CT-like and corticocortical-like neurons more frequently formed compound appositions, where two or more appositions were located on a dendritic branch, than other pyramidal neurons. Layer 6 CT neurons may contribute to intracortical information processing through preferential connections with PV neurons in layers 5b–6.
Page Count	20
Journal	eNeuro
Volume Number	9
PubMed Central reference number	PMC8856719
Issue Number	1
PubMed reference number	34965927
e-ISSN	23732822
DOI	10.1523/ENEURO.0567-20.2021
Language	English
Publisher	Society for Neuroscience
Publisher Date	2022-01-01
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Copyright © 2022 Kuramoto et al.
Subject Keyword	cerebral cortex corticothalamic neurons excitatory neurons layer 6 neurons motor area parvalbumin-positive interneurons
Content Type	Text
Resource Type	Article
Subject	Medicine Neuroscience