A surface protein-imprinted biosensor based on boronate affinity for the detection of anti-human immunoglobulin G.

Content Provider	Europe PMC
Author	Liu, Zixuan Yin, Zheng-Zhi Cai, Wenrong Wu, Datong Li, Junyao Kong, Yong
Abstract	A surface protein-imprinted biosensor was constructed on a screen-printed carbon electrode (SPCE) for the detection of anti-human immunoglobulin G (anti-IgG). The SPCE was successively decorated with aminated graphene (NH2-G) and gold nanobipyramids (AuNBs) for signal amplification. Then 4-mercaptophenylboric acid (4-MPBA) was covalently anchored to the surface of AuNBs for capturing anti-IgG template through boronate affinity binding. The decorated SPCE was then deposited with an imprinting layer generated by the electropolymerization of pyrrole. After removal of the anti-IgG template by the dissociation of the boronate ester in an acidic solution, three-dimensional (3D) cavities complementary to the anti-IgG template were formed in the imprinting layer of polypyrrole (PPy). The molecularly imprinted polymers (MIP)-based biosensor was used for the detection of anti-IgG, exhibiting a wide linear range from 0.05 to 100 ng mL−1 and a low limit of detection of 0.017 ng mL−1 (S/N = 3). In addition, the MIP-based anti-IgG biosensor also shows high selectivity, reproducibility and stability. Finally, the practicability of the fabricated anti-IgG biosensor was demonstrated by accurate determination of anti-IgG in serum sample.Supplementary InformationThe online version contains supplementary material available at 10.1007/s00604-022-05204-w.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC8853174&blobtype=pdf
ISSN	00263672
Journal	Mikrochimica Acta [Mikrochim Acta]
Volume Number	189
DOI	10.1007/s00604-022-05204-w
PubMed Central reference number	PMC8853174
Issue Number	3
PubMed reference number	35166940
e-ISSN	14365073
Language	English
Publisher	Springer Vienna
Publisher Date	2022-02-15
Publisher Place	Vienna
Access Restriction	Open
Rights License	This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2022
Subject Keyword	Molecularly imprinted polymers Surface imprinting Boronate affinity Gold nanobipyramids Anti-IgG
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry