ANXA3 is upregulated by hypoxia-inducible factor 1-alpha and promotes colon cancer growth.

Content Provider	Europe PMC
Author	Du, Kunli Ren, Jiahui Fu, Zhongxue Wu, Xingye Zheng, Jianyong Li, Xing
Copyright Year	2020
Abstract	BackgroundAnnexin A3 (ANXA3) is overexpressed in various cancers and is a potential target for cancer treatment. However, clinical implication and biological function of ANXA3 in colon cancer remain unknown. This study aimed to investigate the relationship between hypoxia-inducible factor 1-alpha (HIF-1α) and ANXA3, and explore the function of ANXA3 in colon carcinoma.MethodsExpression levels of HIF-1α and ANXA3 in human colon carcinoma specimens and colon cancer cell lines were detected by immunohistochemistry, real-time PCR and Western blot analysis. The proliferation of colon cancer cells was examined. Nude mice were used for xenograft tumor model, and HIF-1α siRNA or control adenovirus was injected into the tumor.ResultsHIF-1α and ANXA3 expression levels were higher in colon cancer tissues than their expression levels in normal colon tissues. In addition, HIF-1α and ANXA3 expression increased in colon cancer cells under hypoxic condition. Knockdown of HIF-1α decreased HIF-1α and ANXA3 expression, and inhibited the proliferation and growth of colon cancer cells. In nude mouse model, silencing HIF-1α decreased volume of xenograft tumor and ANXA3 expression.ConclusionsANXA3 expression is upregulated by HIF-1α in colon cancer in response to hypoxic stress and contributes to colon tumor growth. ANXA3 may represent a new therapeutic target for colon carcinoma.
ISSN	2218676X
Journal	Translational Cancer Research
Volume Number	9
PubMed Central reference number	PMC8797770
Issue Number	12
PubMed reference number	35117344
e-ISSN	22196803
DOI	10.21037/tcr-20-994
Language	English
Publisher	AME Publishing Company
Publisher Date	2020-12-01
Access Restriction	Open
Rights License	Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/. 2020 Translational Cancer Research. All rights reserved.
Subject Keyword	Hypoxia-inducible factor 1-alpha (HIF-1α) annexin A3 (ANXA3) colon cancer hypoxia
Content Type	Text
Resource Type	Article
Subject	Radiology, Nuclear Medicine and Imaging Cancer Research Oncology