Loading...
Please wait, while we are loading the content...
Similar Documents
miR-1290 inhibits chordoma cell proliferation and invasion by targeting Robo1.
| Content Provider | Europe PMC |
|---|---|
| Author | Wang, Bin Zhang, Kai Chen, Hao Lu, Jian Wu, Guizhong Yang, Huilin Chen, Kangwu |
| Copyright Year | 2019 |
| Abstract | BackgroundChordoma is a low-grade aggressive bone tumor with a high local recurrence. MicroRNAs (miRNAs) have been reported to play crucial roles in the development of chordoma. Our previous study has shown miR-1290 is associated with muscle invasion and the prognosis of chordoma. However, the underlying mechanism of miR-1290 in chordoma remains unclear. In this study, we aimed to explore the function of miR-1290 in the biological behaviors of chordoma.MethodsSixteen sacral chordoma samples and 10 fetal nucleus pulposus specimens were collected for the detection of miR-1290 and Robo1 at the First Affiliated Hospital of Soochow University. Bioinformatic analysis and a luciferase reporter assay was used to verify the interaction between miR-1290 and the target gene robo1 in chordoma. Effects of miR-1290 expression on chordoma cell proliferation and invasion were explored by clone formation and Transwell assay in vitro. The underlying mechanisms of miR-1290 and Robo1 in chordoma cell proliferation and invasion were also explored in the U-CH1 cell line.ResultsIn vitro functional analysis, including clone formation, and Transwell assays indicated overexpression of miR-1290 significantly suppressed chordoma cell proliferation and invasion. Bioinformatic analysis revealed Robo1 as a potential target of miR-1290, and luciferase reporter assays demonstrated the association between miR-1290 and the Robo1 gene in U-CH1 cells. Robo1 was further confirmed to be up-regulated in chordoma tissues by immunohistochemistry (IHC), which is negatively correlated with miR-1290 expression in chordoma tissue. Additionally, we found down-regulation of miR-1290 could induce the expression of Robo1 in chordoma cells, while the elevation of miR-1290 expression could inhibit Robo1 expression in chordoma cells.ConclusionsmiR-1290 inhibits chordoma cell proliferation and invasion by negatively regulating the Robo1 gene. |
| ISSN | 2218676X |
| Journal | Translational Cancer Research |
| Volume Number | 8 |
| PubMed Central reference number | PMC8797437 |
| Issue Number | 2 |
| PubMed reference number | 35116786 |
| e-ISSN | 22196803 |
| DOI | 10.21037/tcr.2019.03.13 |
| Language | English |
| Publisher | AME Publishing Company |
| Publisher Date | 2019-04-01 |
| Access Restriction | Open |
| Rights License | Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/. 2019 Translational Cancer Research. All rights reserved. |
| Subject Keyword | Chordoma miR-1290 Robo1 cell invasion proliferation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Radiology, Nuclear Medicine and Imaging Cancer Research Oncology |
