Improved chromosome-level genome assembly of the Glanville fritillary butterfly (Melitaea cinxia) integrating Pacific Biosciences long reads and a high-density linkage map.

Content Provider	Europe PMC
Author	Smolander, Olli-Pekka Blande, Daniel Ahola, Virpi Rastas, Pasi Tanskanen, Jaakko Kammonen, Juhana I Oostra, Vicencio Pellegrini, Lorenzo Ikonen, Suvi Dallas, Tad DiLeo, Michelle F Duplouy, Anne Duru, Ilhan Cem Halimaa, Pauliina Kahilainen, Aapo Kuwar, Suyog S Kärenlampi, Sirpa O Lafuente, Elvira Luo, Shiqi Makkonen, Jenny Nair, Abhilash de la Paz Celorio-Mancera, Maria Pennanen, Ville Ruokolainen, Annukka Sundell, Tarja Tervahauta, Arja I Twort, Victoria van Bergen, Erik Österman-Udd, Janina Paulin, Lars Frilander, Mikko J Auvinen, Petri Saastamoinen, Marjo
Copyright Year	2022
Abstract	BackgroundThe Glanville fritillary (Melitaea cinxia) butterfly is a model system for metapopulation dynamics research in fragmented landscapes. Here, we provide a chromosome-level assembly of the butterfly's genome produced from Pacific Biosciences sequencing of a pool of males, combined with a linkage map from population crosses.ResultsThe final assembly size of 484 Mb is an increase of 94 Mb on the previously published genome. Estimation of the completeness of the genome with BUSCO indicates that the genome contains 92–94% of the BUSCO genes in complete and single copies. We predicted 14,810 genes using the MAKER pipeline and manually curated 1,232 of these gene models.ConclusionsThe genome and its annotated gene models are a valuable resource for future comparative genomics, molecular biology, transcriptome, and genetics studies on this species.
Page Count	12
Journal	GigaScience
Volume Number	11
PubMed Central reference number	PMC8756199
Issue Number	1
PubMed reference number	35022701
e-ISSN	2047217X
DOI	10.1093/gigascience/giab097
Language	English
Publisher	Oxford University Press
Publisher Date	2022-01-01
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. © The Author(s) 2022. Published by Oxford University Press GigaScience.
Subject Keyword	Melitaea cinxia Glanville fritillary genome spatial ecology
Content Type	Text
Resource Type	Article
Subject	Computer Science Applications Health Informatics