NDLI: Two different cell-cycle processes determine the timing of cell division in <i>Escherichia coli</i>.

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Two different cell-cycle processes determine the timing of cell division in Escherichia coli.

Content Provider	Europe PMC
Author	Colin, Alexandra Micali, Gabriele Faure, Louis Cosentino Lagomarsino, Marco van Teeffelen, Sven
Editor	Seminara, Agnese Barkai, Naama
Copyright Year	2021
Abstract	Cells must control the cell cycle to ensure that key processes are brought to completion. In Escherichia coli, it is controversial whether cell division is tied to chromosome replication or to a replication-independent inter-division process. A recent model suggests instead that both processes may limit cell division with comparable odds in single cells. Here, we tested this possibility experimentally by monitoring single-cell division and replication over multiple generations at slow growth. We then perturbed cell width, causing an increase of the time between replication termination and division. As a consequence, replication became decreasingly limiting for cell division, while correlations between birth and division and between subsequent replication-initiation events were maintained. Our experiments support the hypothesis that both chromosome replication and a replication-independent inter-division process can limit cell division: the two processes have balanced contributions in non-perturbed cells, while our width perturbations increase the odds of the replication-independent process being limiting.
Journal	eLife
Volume Number	10
PubMed Central reference number	PMC8555983
PubMed reference number	34612203
e-ISSN	2050084X
DOI	10.7554/elife.67495
Language	English
Publisher	eLife Sciences Publications, Ltd
Publisher Date	2021-10-06
Access Restriction	Open
Rights License	This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. © 2021, Colin et al
Subject Keyword	cell cycle control cell division chromosome replication single-cell correlations live-cell microscopy theoretical modeling E. coli
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Neuroscience Medicine Biochemistry, Genetics and Molecular Biology

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