Drug-Free Enzyme-Based Bactericidal Nanomotors against Pathogenic Bacteria.

Content Provider	Europe PMC
Author	Vilela, Diana Blanco-Cabra, Nuria Eguskiza, Ander Hortelao, Ana C. Torrents, Eduard Sanchez, Samuel
Copyright Year	2021
Abstract	Thelow efficacy of current conventional treatments for bacterialinfections increases mortality rates worldwide. To alleviate thisglobal health problem, we propose drug-free enzyme-based nanomotorsfor the treatment of bacterial urinary-tract infections. We developnanomotors consisting of mesoporous silica nanoparticles (MSNPs) thatwere functionalized with either urease (U-MSNPs), lysozyme (L-MSNPs),or urease and lysozyme (M-MSNPs), and use them against nonpathogenicplanktonic Escherichia coli. U-MSNPsexhibited the highest bactericidal activity due to biocatalysis ofurea into NaHCO3 and NH3, which also propelsU-MSNPs. In addition, U-MSNPs in concentrations above 200 μg/mLwere capable of successfully reducing 60% of the biofilm biomass ofa uropathogenic E. coli strain. Thisstudy thus provides a proof-of-concept, demonstrating that enzyme-basednanomotors are capable of fighting infectious diseases. This approachcould potentially be extended to other kinds of diseases by selectingappropriate biomolecules.
ISSN	19448244
Journal	ACS Applied Materials & Interfaces
Volume Number	13
PubMed Central reference number	PMC8478280
Issue Number	13
PubMed reference number	33769023
e-ISSN	19448252
DOI	10.1021/acsami.1c00986
Language	English
Publisher	AmericanChemical Society
Publisher Date	2021-03-26
Access Restriction	Open
Rights License	Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). © 2021 American ChemicalSociety
Subject Keyword	enzymatic nanomotors biofilms E. coli infections nanomachines self-propulsion
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Materials Science