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Hydration Layer of Only a Few Molecules Controls Lipid Mobility in Biomimetic Membranes.
| Content Provider | Europe PMC |
|---|---|
| Author | Chattopadhyay, Madhurima Krok, Emilia Orlikowska, Hanna Schwille, Petra Franquelim, Henri G. Piatkowski, Lukasz |
| Copyright Year | 2021 |
| Abstract | Self-assembly ofbiomembranes results from the intricate interactionsbetween water and the lipids’ hydrophilic head groups. Therefore,the lipid–water interplay strongly contributes to modulatingmembrane architecture, lipid diffusion, and chemical activity. Here,we introduce a new method of obtaining dehydrated, phase-separated,supported lipid bilayers (SLBs) solely by controlling the decreaseof their environment’s relative humidity. This facilitatesthe study of the structure and dynamics of SLBs over a wide rangeof hydration states. We show that the lipid domain structure of phase-separatedSLBs is largely insensitive to the presence of the hydration layer.In stark contrast, lipid mobility is drastically affected by dehydration,showing a 6-fold decrease in lateral diffusion. At the same time,the diffusion activation energy increases approximately 2-fold forthe dehydrated membrane. The obtained results, correlated with thehydration structure of a lipid molecule, revealed that about six toseven water molecules directly hydrating the phosphocholine moietyplay a pivotal role in modulating lipid diffusion. These findingscould provide deeper insights into the fundamental reactions wherelocal dehydration occurs, for instance during cell–cell fusion,and help us better understand the survivability of anhydrobiotic organisms.Finally, the strong dependence of lipid mobility on the number ofhydrating water molecules opens up an application potential for SLBsas very precise, nanoscale hydration sensors. |
| ISSN | 00027863 |
| Volume Number | 143 |
| PubMed Central reference number | PMC8447254 |
| Issue Number | 36 |
| PubMed reference number | 34342967 |
| Journal | Journal of the American Chemical Society [J. Am. Chem. Soc] |
| e-ISSN | 15205126 |
| DOI | 10.1021/jacs.1c04314 |
| Language | English |
| Publisher | American Chemical Society |
| Publisher Date | 2021-08-03 |
| Access Restriction | Open |
| Rights License | Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). © 2021 The Authors. Published by American Chemical Society |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Colloid and Surface Chemistry Biochemistry Catalysis |
