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Performance assessment of seven SARS-CoV-2 IgG enzyme-linked immunosorbent assays.
| Content Provider | Europe PMC |
|---|---|
| Author | Deshpande, Ketki PT, Ullas Kaduskar, Ojas Vijay, Neetu Rakhe, Aparna Vidhate, Shankar Khutwad, Kirtee Deshpande, Gururaj Rao Tilekar, Bipin Saka, Sanskruti Gadekar, Kshitija Patil, Roshni Yadav, Pragya Potdar, Varsha Gurav, Yogesh Gupta, Priyanka Kaur, Harmanmeet Narayan, Jitendra Sapkal, Gajanan Abraham, Priya |
| Abstract | The pandemic of COVID‐19 has caused enormous fatalities worldwide. Serological assays are important for detection of asymptomatic or mild cases of COVID‐19, and sero‐prevalence and vaccine efficacy studies. Here, we evaluated and compared the performance of seven commercially available enzyme‐linked immunosorbent assay (ELISA)s for detection of anti‐severe acute respiratory syndrome corona virus 2 (SARS‐CoV‐2) immunoglobulin G (IgG). The ELISAs were evaluated with a characterized panel of 100 serum samples from qRT‐PCR confirmed COVID‐19 patients, collected 14 days post onset disease, 100 SARS‐CoV‐2 negative samples and compared the results with that of neutralization assay. Results were analysed by creating the receiver operating characteristic curve of all the assays in reference to the neutralization assay. All kits, were found to be suitable for detection of IgG against SARS‐CoV‐2 with high accuracy. The DiaPro COVID‐19 IgG ELISA showed the highest sensitivity (98%) among the kits. The assays demonstrated high sensitivity and specificity in detecting the IgG antibodies against SARS‐CoV‐2. However, the presence of IgG antibodies does not always correspond to neutralizing antibodies. Due to their good accuracy indices, these assays can also aid in tracing mild infections, in cohort studies and in pre‐vaccine evaluations. |
| Page Count | 7 |
| ISSN | 01466615 |
| Journal | Journal of Medical Virology |
| Volume Number | 93 |
| PubMed Central reference number | PMC8426713 |
| Issue Number | 12 |
| PubMed reference number | 34331713 |
| e-ISSN | 10969071 |
| DOI | 10.1002/jmv.27251 |
| Language | English |
| Publisher | John Wiley and Sons Inc. |
| Publisher Date | 2021-08-10 |
| Publisher Place | Hoboken |
| Access Restriction | Open |
| Rights License | This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency. © 2021 Wiley Periodicals LLC |
| Subject Keyword | COVID‐19 ELISA IgG antibody assay ROC SARS‐CoV‐2 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Virology |
