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The use of a novel deer antler decellularized cartilage-derived matrix scaffold for repair of osteochondral defects.
| Content Provider | Europe PMC |
|---|---|
| Author | Chu, Wenhui Hu, Gaowei Peng, Lin Zhang, Wei Ma, Zhe |
| Abstract | BackgroundThe physiologic regenerative capacity of cartilage is severely limited. Current studies on the repair of osteochondral defects (OCDs) have mainly focused on the regeneration of cartilage tissues. The antler cartilage is a unique regenerative cartilage that has the potential for cartilage repair.MethodsAntler decellularized cartilage-derived matrix scaffolds (adCDMs) were prepared by combining freezing-thawing and enzymatic degradation. Their DNA, glycosaminoglycans (GAGs), and collagen content were then detected. Biosafety and biocompatibility were evaluated by pyrogen detection, hemolysis analysis, cytotoxicity evaluation, and subcutaneous implantation experiments. adCDMs were implanted into rabbit articular cartilage defects for 2 months to evaluate their therapeutic effects.ResultsAdCDMs were observed to be rich in collagen and GAGs and devoid of cells. AdCDMs were also determined to have good biosafety and biocompatibility. Both four- and eight-week treatments of OCDs showed a flat and smooth surface of the healing cartilage at the adCDMs filled site. The international cartilage repair society scores (ICRS) of adCDMs were significantly higher than those of controls (porcine dCDMs and normal saline) (p < 0.05). The repaired tissue in the adCDM group was fibrotic with high collagen, specifically, type II collagen.ConclusionsWe concluded that adCDMs could achieve excellent cartilage regeneration repair in a rabbit knee OCDs model. Our study stresses the importance and benefits of adCDMs in bone formation and overall anatomical reconstitution, and it provides a novel source for developing cartilage-regenerating repair materials.Supplementary InformationThe online version contains supplementary material available at 10.1186/s13036-021-00274-5. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC8414868&blobtype=pdf |
| Journal | Journal of Biological Engineering [J Biol Eng] |
| Volume Number | 15 |
| DOI | 10.1186/s13036-021-00274-5 |
| PubMed Central reference number | PMC8414868 |
| Issue Number | 1 |
| PubMed reference number | 34479610 |
| e-ISSN | 17541611 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2021-09-03 |
| Publisher Place | London |
| Access Restriction | Open |
| Rights License | Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. © The Author(s) 2021 |
| Subject Keyword | Decellularization Antler cartilage Cartilage-derived matrix scaffolds Tissue engineering Osteochondral defect |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Environmental Engineering Molecular Biology Biomedical Engineering |
