Enriching Acidophilic Fe(II)-oxidizing Bacteria in No-flow, Fed-batch Systems.

Content Provider	Europe PMC
Author	Sheng, Yizhi Kaley, Bradley Burgos, William D.
Copyright Year	2017
Abstract	Low-pH microbial Fe(II) oxidation occurs naturally in some Fe(II)-rich acid mine drainage (AMD) ecosystems across so-called terraced iron formations. Indigenous acidophilic Fe(II)-oxidizing bacterial communities can be incorporated into both passive and active treatments to remove Fe from the AMD solution. Here, we present a protocol of enriching acidophilic Fe(II)-oxidizing bacteria in no-flow, fed-batch systems. Mixed cultures of naturally occurring microbes are enriched from the fresh surface sediments at AMD sites using a chemo-static bioreactor (Eppendorf BioFlo®/Celligen® 115 Fermentor) with respect to constant stirring speed, temperature, pH and unlimited dissolved oxygen. Ferrous sulfate is discontinuously added to the reactor as the primary substrate to enrich for acidophilic Fe(II)-oxidizing bacteria. Successfully and efficiently enriching acidophilic Fe(II)-oxidizing bacteria helps to exploit this biogeochemical process into AMD treatment systems.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC8376609&blobtype=pdf
Volume Number	7
PubMed Central reference number	PMC8376609
Issue Number	3
PubMed reference number	34458451
Journal	Bio-protocol [Bio Protoc]
e-ISSN	23318325
DOI	10.21769/bioprotoc.2130
Language	English
Publisher	Bio-Protocol
Publisher Date	2017-02-05
Publisher Place	1075 Lorne Way, Sunnyvale, CA 94087, USA
Access Restriction	Open
Rights License	Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.
Subject Keyword	Acid mine drainage Enrichment Bioreactor Fe(II)-oxidizing bacteria Bioremediation
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Neuroscience Plant Science Biochemistry, Genetics and Molecular Biology