Rearing of Hyalomma marginatum (Acarina: Ixodidae) under laboratory conditions in Morocco.

Content Provider	Europe PMC
Author	Elhachimi, Latifa Valcárcel, Félix Olmeda, Angeles S. Elasatey, Sabrine Khattat, Sarah E. Daminet, Sylvie Sahibi, Hamid Duchateau, Luc
Abstract	Hyalomma marginatum Koch is one of the main tick vectors of human and animal tick-borne diseases. The objective of this study was to establish standard procedures for rearing H. marginatum under laboratory conditions. Such laboratory tick populations are required to study acaricide resistance of Hyalomma ticks. In our rearing program, larvae and nymphs were fed on New Zealand white rabbits, whereas adults were fed on sheep. Non-parasitic stages were held at 18 and 28 °C to study the effect of temperature on development and survival. In our experiments, H. marginatum ticks have maintained the characteristics of a two-host life cycle. The engorged larvae did not detach and moulted on the rabbit, after which the emerged nymphs continued to feed on the same animal. The life cycle duration of H. marginatum was influenced by temperature, with each non-parasitic stage—i.e., larva and nymph molting—developing faster at 28 than at 18 °C; preoviposition and oviposition periods were shorter at 28 than at 18 °C. At 18 °C, no eggs hatched. The whole cycle from the collection of an engorged field tick until the emergence of second-generation larvae took 189 days. One such tick on average results in 3500 eggs which over time, taking into account the losses at each developmental stage, develop into 1200 adult ticks. Rearing these ticks a second generation therefore could result in millions of larval ticks.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC8367888&blobtype=pdf
ISSN	01688162
Journal	Experimental & Applied Acarology [Exp Appl Acarol]
Volume Number	84
DOI	10.1007/s10493-021-00641-3
PubMed Central reference number	PMC8367888
Issue Number	4
PubMed reference number	34231094
e-ISSN	15729702
Language	English
Publisher	Springer International Publishing
Publisher Date	2021-07-06
Publisher Place	Gewerbestrasse 11, Cham, Ch 6330, Switzerland
Access Restriction	Open
Rights License	Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2021
Subject Keyword	Hyalomma marginatum Morocco Rearing Life cycle time Temperature
Content Type	Text
Resource Type	Article
Subject	Ecology Medicine Insect Science