Circulating Tumor Cells Expressing Krüppel-Like Factor 8 and Vimentin as Predictors of Poor Prognosis in Pancreatic Cancer Patients.

Content Provider	Europe PMC
Author	Zhu, Peng Liu, Hui-Ying Liu, Fu-Chen Gu, Fang-Ming Yuan, Sheng-Xian Huang, Jian Pan, Ze-Ya Wang, Wei-Jun
Copyright Year	2021
Abstract	Background:Circulating tumor cells (CTCs) with an epithelial-mesenchymal transition phenotype in peripheral blood may be a useful marker of carcinomas with poor prognosis. The aim of this study was to determine the prognostic significance of CTCs expressing Krüppel-like factor 8 (KLF8) and vimentin in pancreatic cancer (PC).Methods:CTCs were isolated by immunomagnetic separation from the peripheral blood of 40 PC patients before undergoing surgical resection. Immunocytochemistry was performed to identify KLF8+ and vimentin+ CTCs. The associations between CTCs and time to recurrence (TTR), clinicopathologic factors, and survival were assessed. Univariate and multivariate analyzes were performed to identify risk factors.Results:Patients with CTCs (n = 30) had a higher relapse rate compared to those without (n = 10) (70.0% vs 20.0%; P < 0.01). The proportion of KLF8+/vimentin+ CTCs to total CTCs was inversely related to TTR (r = −0.646; P < 0.01); TTR was reduced in patients with > 50% of CTCs identified as KLF8+/vimentin+ (P < 0.01). Independent risk factors for recurrence were perineural invasion and > 50% KLF8+/vimentin+ CTCs (both P < 0.05).Conclusion:Poor prognosis can be predicted in PC patients when > 50% of CTCs are positive for KLF8 and vimentin.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC8361509&blobtype=pdf
ISSN	10732748
Journal	Cancer Control : Journal of the Moffitt Cancer Center [Cancer Control]
Volume Number	28
DOI	10.1177/10732748211027163
PubMed Central reference number	PMC8361509
PubMed reference number	34378430
e-ISSN	15262359
Language	English
Publisher	SAGE Publications
Publisher Date	2021-01-01
Publisher Place	Sage CA: Los Angeles, CA
Access Restriction	Open
Rights License	This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). © The Author(s) 2021
Subject Keyword	circulating tumor cells Krüppel-like factor 8 pancreatic cancer prognosis recurrence vimentin
Content Type	Text
Resource Type	Article
Subject	Hematology Oncology