Evaluation of automated antigen detection test for detection of SARS-CoV-2.

Content Provider	Europe PMC
Author	Mak, Gannon C.K. Lau, Stephen S.Y. Wong, Kitty K.Y. Chow, Nancy L.S. Lau, C.S. Ng, Ken H.L. Lam, Edman T.K. Chan, Rickjason C.W. Tsang, Dominic N.C.
Copyright Year	2021
Abstract	RT-PCR is the gold standard to detect SARS-CoV-2, however, its capacity is limited. We evaluated an automated antigen detection (AAD) test, Elecsys SARS-CoV-2 Antigen (Roche, Germany), for detecting SARS-CoV-2. We compared the limit of detection (LOD) between AAD test, rapid antigen detection (RAD) test; SARS-CoV-2 Rapid Antigen Test (SD Biosensor, Korea), and in-house RT-PCR test. LOD results showed that the AAD test was 100 fold more sensitive than the RAD test, while the sensitivity of the AAD test was comparable to the RT-PCR test. The AAD test detected between 85.7% and 88.6% of RT-PCR-positive specimens collected from COVID-19 patients, false negative results were observed for specimens with Ct values >30. Although clinical sensitivity for the AAD test was not superior or comparable to the RT-PCR test in the present study, the AAD test may be an alternative to RT-PCR test in terms of turn-around time and throughput.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC8284070&blobtype=pdf
ISSN	07328893
Journal	Diagnostic Microbiology and Infectious Disease [Diagn Microbiol Infect Dis]
Volume Number	101
DOI	10.1016/j.diagmicrobio.2021.115490
PubMed Central reference number	PMC8284070
Issue Number	4
PubMed reference number	34399380
e-ISSN	18790070
Language	English
Publisher	Elsevier Inc.
Publisher Date	2021-07-16
Access Restriction	Open
Rights License	Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. © 2021 Elsevier Inc. All rights reserved.
Subject Keyword	SARS-CoV-2 COVID-19 Automated Antigen Detection Rapid Antigen Detection RT-PCR
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology (medical)