Nrf2 Pathway Ameliorates Bladder Dysfunction in Cyclophosphamide-Induced Cystitis via Suppression of Oxidative Stress.

Content Provider	Europe PMC
Author	Ni, Bin Chen, Zhengsen Shu, Le Shao, Yunpeng Huang, Yi Tamrat, Nebiyu Elias Wei, Zhongqing Shen, Baixin
Copyright Year	2021
Abstract	Objective To investigate the protective effect and molecular mechanism of nuclear factor E2-related factor 2 (Nrf2) pathway in interstitial cystitis (IC). Methods We established a mouse model of IC by cyclophosphamide (CYP) in wild-type mice and Nrf2 gene knockout mice. We examined the histological and functional alterations, the changes of oxidative stress markers, and the expression of the antioxidant genes downstream of Nrf2 pathway. Results After CYP administration, the mice showed urinary frequency and urgency, pain sensitization, decreased contractility, bladder edema, and oxidative stress disorder. Notably, the Nrf2−/− CYP mice had more severe symptoms. The mRNA and protein levels of antioxidant genes downstream of Nrf2 pathway were significantly upregulated in the Nrf2+/+ CYP mice, while there were no significant changes in the Nrf2−/− CYP mice. Conclusion Nrf2 pathway protects bladder injury and ameliorates bladder dysfunction in IC, possibly by upregulating antioxidant genes and inhibiting oxidative stress.
ISSN	19420900
Journal	Oxidative Medicine and Cellular Longevity
Volume Number	2021
PubMed Central reference number	PMC8279868
PubMed reference number	34306306
e-ISSN	19420994
DOI	10.1155/2021/4009308
Language	English
Publisher	Hindawi
Publisher Date	2021-06-30
Access Restriction	Open
Rights License	This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2021 Bin Ni et al.
Content Type	Text
Resource Type	Article
Subject	Cell Biology Aging Medicine Biochemistry