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Black cardamom essential oil prevents Escherichia coli O157:H7 and Salmonella Typhimurium JSG 1748 biofilm formation through inhibition of quorum sensing.
| Content Provider | Europe PMC |
|---|---|
| Author | Algburi, Ammar Asghar, Ali Huang, Qingrong Mustfa, Warda Javed, Hafiz U. Zehm, Saskia Chikindas, Michael L. Abdullah |
| Abstract | This study aimed to investigate the chemical composition, using GC–MS, and anti-biofilm potential of black cardamom essential oil (BCEO) against biofilms of Escherichia coli O157:H7 and Salmonella Typhimurium JSG 1748 through inhibition of bacterial quorum sensing. GC–MS quantification demonstrated that BCEO contains 1,8-cineole (44.24%), α-terpinyl acetate (12.25%), nerolidol (6.03%), and sabinene (5.96%) as the major bioactive compounds. Antioxidant assays for BCEO revealed the total phenolic and flavonoid mean values were 1325.03 ± 7.69 mg GAE 100/g and 168.25 ± 5.26 mg CE/g, respectively. In regards to antimicrobial potential, Candida albicans was the most sensitive species compared to Streptococcus mutans, Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus, and Salmonella Typhimurium with the following zones of inhibition; 14.4 ± 0.52, 13.2 ± 0.42, 11.2 ± 0.28, 11.0 ± 0.52, 8.2 ± 0.24 and 6.6 ± 0.18 mm in diameter, respectively. Biofilm inhibition by BCEO was concentration-dependent, when various concentrations of 0.03, 0.06, 0.12, 0.25 and 0.5% were applied, 33.67, 34.14, 38.66, 46.65 and 50.17% of Salmonella Typhimurium biofilm was inhibited, while 47.31, 54.15, 76.57, 83.36 and 84.63% of Escherichia coli biofilm formation was prevented. Chromobacterium violaceum ATCC 12,472 and its product violacein, was used as a microbial indicator for enhancement or inhibition of quorum sensing. Our data showed that 0.5% of BCEO inhibited violacein production without influencing the growth of Chromobacterium violaceum, while 1% of BCEO, caused 100% inhibtion of violacein production together with 30% inhibition of growth. This study shows that BCEO possesses promising antioxidant and antimicrobial potential, and found anti-biofilm activities linked to the quenching of the quorum sensing system of E. coli and S. Typhimurium. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC8249550&blobtype=pdf |
| ISSN | 00221155 |
| Volume Number | 58 |
| DOI | 10.1007/s13197-020-04821-8 |
| PubMed Central reference number | PMC8249550 |
| Issue Number | 8 |
| PubMed reference number | 34294980 |
| Journal | Journal of Food Science and Technology [J Food Sci Technol] |
| e-ISSN | 09758402 |
| Language | English |
| Publisher | Springer India |
| Publisher Date | 2020-10-01 |
| Publisher Place | New Delhi |
| Access Restriction | Open |
| Rights License | © Association of Food Scientists & Technologists (India) 2020 |
| Subject Keyword | Black cardamom essential oil GC–MS Antioxidants Anti-biofilm Quorum sensing inhibition |
| Content Type | Text |
| Resource Type | Article |
| Subject | Food Science |
