Loading...
Please wait, while we are loading the content...
Similar Documents
Quantification and Imaging of Nanoscale Contact with Förster Resonance Energy Transfer.
| Content Provider | Europe PMC |
|---|---|
| Author | Simões, MónicaG. Urstöger, Georg Schennach, Robert Hirn, Ulrich |
| Copyright Year | 2021 |
| Abstract | Adhesion is causedby molecular interactions that only take placeif the surfaces are in nanoscale contact (NSC); i.e., the distancebetween the surfaces is in the range of 0.1–0.4 nm. However,there are several difficulties measuring the NSC between surfaces,mainly because regions that appear to be in full contact at low magnificationmay show no NSC when observed at higher magnifications. Thus, themeasurement area of NSC is very small with imaging techniques, andan experimental technique to evaluate NSC for large contact areashas not been available thus far. Here, we are proposing Försterresonance energy transfer (FRET) spectroscopy/microscopy for thispurpose. We demonstrate that NSC in a distance range of 1–10nm can be evaluated. Our experiments reveal that, for thin films pressedunder different loads, NSC increases with the applied pressure, resultingin a higher FRET signal and a corresponding increase in adhesion force/energywhen separating the films. Furthermore, we show that local variationsin molecular contact can be visualized with FRET microscopy. Thus,we are introducing a spectroscopic technique for quantification (FRETspectroscopy) and imaging (FRET microscopy) of NSC between surfaces,demonstrated here for the application of surface adhesion. This couldbe of interest for all fields where adhesion or nanoscale surfacecontact are playing a role, for example, soft matter, biological materials,and polymers, but also engineering applications, like tribology, adhesives,and sealants. |
| ISSN | 19448244 |
| Journal | ACS Applied Materials & Interfaces |
| Volume Number | 13 |
| PubMed Central reference number | PMC8153545 |
| Issue Number | 16 |
| PubMed reference number | 33856765 |
| e-ISSN | 19448252 |
| DOI | 10.1021/acsami.1c04226 |
| Language | English |
| Publisher | AmericanChemical Society |
| Publisher Date | 2021-04-15 |
| Access Restriction | Open |
| Rights License | Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). © 2021 TheAuthors. Published by AmericanChemical Society |
| Subject Keyword | nanoscale contact adhesion contact mechanics Försterresonance energy transfer polymer films FRET spectroscopy FRET microscopy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Materials Science |
