Loading...
Please wait, while we are loading the content...
Similar Documents
The long noncoding RNA THRIL knockdown protects hypoxia-induced injuries of H9C2 cells through regulating miR-99a.
| Content Provider | Europe PMC |
|---|---|
| Author | Xia, Jingwen Jiang, Nianxin Li, Yansong Wei, Yong Zhang, Xuan |
| Copyright Year | 2019 |
| Abstract | BackgroundMyocardial infarction (MI) is a leading cause of disease with high morbidity and mortality worldwide. Recent studies have revealed that long non-coding RNAs (lncRNAs) are involved in heart disease pathogenesis. This study aimed to investigate the effect and the molecular basis of THRIL on hypoxia-injured H9C2 cells.MethodsTHRIL, miR-99a and Brahma-related gene 1 (Brg1) expressions in H9C2 cells were altered by transient transfections. The cells were subjected to hypoxia for 4 h, and then the levels of THRIL, miR-99a and Brg1 were investigated. Cell viability, migration and invasion, and apoptotic cells were respectively measured by trypan blue exclusion assay, transwell migration assay and flow cytometry assay. Dual luciferase reporter assay was conducted to verify the interaction between miR-99a and THRIL. Furthermore, levels of apoptosis-, PI3K/AKT and mTOR pathways-related factors were measured by western blotting.ResultsHypoxia induced an increase of THRIL but a reduction of miR-99a and Brg1. THRIL inhibition significantly attenuated hypoxia-induced cell injuries, as increased cell viability, migration and invasion, and decreased cell apoptosis. THRIL negatively regulated miR-99a expression through sponging with miR-99a binding site, and miR-99a inhibition abolished the protective effects of THRIL knockdown against hypoxia-induced injury in H9C2 cells. Furthermore, miR-99a positively regulated the expression of Brg1. Brg1 inhibition promoted hypoxia-induced cell injuries, while Brg1 overexpression alleviated hypoxia-induced cell injuries. Moreover, Brg1 overexpression activated PI3K/AKT and mTOR pathways.ConclusionsThis study demonstrated that THRIL inhibition represented a protective effect against hypoxia-induced injuries in H9C2 cells by up-regulating miR-99a expression. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC8084388&blobtype=pdf |
| ISSN | 18975593 |
| Journal | Cardiology Journal [Cardiol J] |
| Volume Number | 26 |
| PubMed Central reference number | PMC8084388 |
| Issue Number | 5 |
| PubMed reference number | 29745968 |
| e-ISSN | 1898018X |
| DOI | 10.5603/cj.a2018.0054 |
| Language | English |
| Publisher | Via Medica |
| Publisher Date | 2018-05-10 |
| Access Restriction | Open |
| Rights License | This article is available in open access under Creative Common Attribution-Non-Commercial-No Derivatives 4.0 International (CC BY-NC-ND 4.0) license, allowing to download articles and share them with others as long as they credit the authors and the publisher, but without permission to change them in any way or use them commercially. Copyright © 2019 Via Medica |
| Subject Keyword | THRIL miR-99a Brg1 myocardial infarction hypoxia |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cardiology and Cardiovascular Medicine |
