Gene Expression Patterns of Cutaneous Squamous Cell Carcinoma and Actinic Keratosis: Biomarkers Screening for Skin Disease Diagnosis.

Content Provider	Europe PMC
Author	Li, Xiaojing Li, Zhifeng Han, Zhao Zhang, Ling Liu, Zhao Liu, Baoguo
Copyright Year	2018
Abstract	BackgroundActinic keratosis (AK) was an intraepidermal tumor which caused by ultraviolet irradiation-induced skin damage.ObjectiveThe aim was to screen biomarkers for development of skin disease by comparing the gene expression profiles between cutaneous squamous cell carcinoma (CSCC) and AK.MethodsGSE45216 with 30 cutaneous squamous cell carcinoma patients and 10 actinic keratosis patients were downloaded and significance analysis of microarrays was processed to screen differently expressed genes (DEGs). Fisher's exact test was processed for DEGs enrichment. Pathway relationship network systematically reflected the signal conduction and synergism between enriched pathways based on Kyoto Encyclopedia of Genes and Genomes database. Gene co-expression network was constructed according to gene expression data. Quantitative real-time-PCR was used to verify screened biomarkers.ResultsTotal 410 DEGs were screened and enriched into various functions, such as signal transduction and negative regulation of apoptotic process. They also participated into cytokine-cytokine receptor interaction and focal adhesion. The pathway relationship network was constructed with 27 nodes. Hub nodes with higher degree of this network were mitogen-activated protein kinase signaling pathway and apoptosis. The gene co-expression network was constructed with 39 nodes. Thereinto, hub node was ELOVL fatty acid elongase. The expression levels of ELOVL4 and HPGD were significantly higher in CSCC samples than that in AK samples, while the expression levels of INHBA and LAMC2 in CSCC samples were significantly lower than that in AK samples.ConclusionThese screened genes, including ELOVL4, HPGD, INHBA and LAMC2, played important roles in transformation from AK to CSCC.
ISSN	10139087
Journal	Annals of Dermatology
Volume Number	30
PubMed Central reference number	PMC7992476
Issue Number	5
PubMed reference number	33911475
e-ISSN	20053894
DOI	10.5021/ad.2018.30.5.536
Language	English
Publisher	The Korean Dermatological Association; The Korean Society for Investigative Dermatology
Publisher Date	2018-08-28
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2018 The Korean Dermatological Association and The Korean Society for Investigative Dermatology
Subject Keyword	Actinic keratosis Diagnosis Genes Neoplasms
Content Type	Text
Resource Type	Article
Subject	Dermatology