Backbone chemical shift spectral assignments of SARS coronavirus-2 non-structural protein nsp9.

Content Provider	Europe PMC
Author	F. Dudás, Erika Puglisi, Rita Korn, Sophie Marianne Alfano, Caterina Bellone, Maria Laura Piaz, Fabrizio Dal Kelly, Geoff Monaca, Elisa Schlundt, Andreas Schwalbe, Harald Pastore, Annalisa
Abstract	As part of an International consortium aiming at the characterization by NMR of the proteins of the SARS-CoV-2 virus, we have obtained the virtually complete assignment of the backbone atoms of the non-structural protein nsp9. This small (12 kDa) protein is encoded by ORF1a, binds to RNA and seems to be essential for viral RNA synthesis. The crystal structures of the SARS-CoV-2 protein and other homologues suggest that the protein is dimeric as also confirmed by analytical ultracentrifugation and dynamic light scattering. Our data constitute the prerequisite for further NMR-based characterization, and provide the starting point for the identification of small molecule lead compounds that could interfere with RNA binding and prevent viral replication.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7985572&blobtype=pdf
ISSN	18742718
Journal	Biomolecular Nmr Assignments [Biomol NMR Assign]
Volume Number	15
DOI	10.1007/s12104-021-10011-0
PubMed Central reference number	PMC7985572
Issue Number	2
PubMed reference number	33755914
e-ISSN	1874270X
Language	English
Publisher	Springer Netherlands
Publisher Date	2021-03-23
Publisher Place	Dordrecht
Access Restriction	Open
Rights License	Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2021
Subject Keyword	Coronavirus Covid-19 NMR Protein SARS-CoV-2 Solution NMR Structure
Content Type	Text
Resource Type	Article
Subject	Structural Biology Biochemistry