¹H^N, ¹³C, and ¹⁵N resonance assignments of the Clostridioides difficile receptor binding domain 2 (CDTb, residues 757-876).

Content Provider	Europe PMC
Author	Cook, Mary E. Varney, Kristen M. Godoy-Ruiz, Raquel Weber, David J.
Abstract	Clostridioides difficile is a bacterial pathogen responsible for the majority of nosocomial infections in the developed world. C. difficile infection (CDI) is difficult to treat in many cases because hypervirulent strains have evolved that contain a third toxin, termed the C. difficile toxin (CDT), in addition to the two enterotoxins TcdA and TcdB. CDT is a binary toxin comprised of an enzymatic, ADP-ribosyltransferase (ART) toxin component, CDTa, and a pore-forming or delivery subunit, CDTb. In the absence of CDTa, CDTb assembles into two distinct di-heptameric states, a symmetric and an asymmetric form with both states having two surface-accessible host cell receptor-binding domains, termed RBD1 and RBD2. RBD1 has a unique amino acid sequence, when aligned to other well-studied binary toxins (i.e., anthrax), and it contains a novel Ca2+-binding site important for CDTb stability. The other receptor binding domain, RBD2, is critically important for CDT toxicity, and a domain such as this is missing altogether in other binary toxins and shows further that CDT is unique when compared to other binary toxins. In this study, the 1H, 13C, and 15N backbone and sidechain resonances of the 120 amino acid RBD2 domain of CDTb (residues 757–876) were assigned sequence-specifically and provide a framework for future NMR-based drug discovery studies directed towards targeting the most virulent strains of CDI.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7973916&blobtype=pdf
ISSN	18742718
Journal	Biomolecular Nmr Assignments [Biomol NMR Assign]
Volume Number	15
DOI	10.1007/s12104-020-09979-y
PubMed Central reference number	PMC7973916
Issue Number	1
PubMed reference number	33034833
e-ISSN	1874270X
Language	English
Publisher	Springer Netherlands
Publisher Date	2020-10-09
Publisher Place	Dordrecht
Access Restriction	Open
Rights License	Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2020
Subject Keyword	Clostridioides difficile CDI CDTb Binary toxin
Content Type	Text
Resource Type	Article
Subject	Structural Biology Biochemistry