microRNA-130b downregulation potentiates chondrogenic differentiation of bone marrow mesenchymal stem cells by targeting SOX9.

Content Provider	Europe PMC
Author	Zhang, Penggui Gao, Guangming Zhou, Ziyu He, Xuejun
Abstract	Osteoarthritis (OA) is a chronic health condition. MicroRNAs (miRs) are critical in chondrocyte apoptosis in OA. We aimed to investigate the mechanism of miR-130b in OA progression. Bone marrow mesenchymal stem cells (BMSCs) and chondrocytes were first extracted. Chondrogenic differentiation of BMSCs was carried out and verified. Chondrocytes were stimulated with interleukin (IL)-1β to imitate OA condition in vitro. The effect of miR-130b on the viability, inflammation, apoptosis, and extracellular matrix of OA chondrocytes was studied. The target gene of miR-130b was predicted and verified. Rescue experiments were performed to further study the underlying downstream mechanism of miR-130b in OA. miR-130b first increased and drastically reduced during chondrogenic differentiation of BMSCs and in OA chondrocytes, respectively, while IL-1β stimulation resulted in increased miR-130b expression in chondrocytes. miR-130b inhibitor promoted chondrogenic differentiation of BMSCs and chondrocyte growth and inhibited the levels of inflammatory factors. miR-130b targeted SOX9. Overexpression of SOX9 facilitated BMSC chondrogenic differentiation and chondrocyte growth, while siRNA-SOX9 contributed to the opposite trends. Silencing of SOX9 significantly attenuated the pro-chondrogenic effects of miR-130b inhibitor on BMSCs. Overall, miR-130b inhibitor induced chondrogenic differentiation of BMSCs and chondrocyte growth by targeting SOX9.
ISSN	0100879X
Journal	Brazilian Journal of Medical and Biological Research
Volume Number	54
PubMed Central reference number	PMC7894390
Issue Number	4
PubMed reference number	33624729
e-ISSN	1414431X
DOI	10.1590/1414-431X202010345
Language	English
Publisher	Associação Brasileira de Divulgação Científica
Publisher Date	2021-02-12
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Subject Keyword	Osteoarthritis miR-130b SOX9 Chondrogenic differentiation Bone marrow mesenchymal stem cells
Content Type	Text
Resource Type	Article
Subject	Neuroscience Cell Biology Physiology Medicine Biochemistry Biophysics Immunology Pharmacology, Toxicology and Pharmaceutics