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Elasticity-dependent response of malignant cells to viscous dissipation.
| Content Provider | Europe PMC |
|---|---|
| Author | Charrier, Elisabeth E. Pogoda, Katarzyna Li, Robin Wells, Rebecca G. Janmey, Paul A. |
| Abstract | The stiffness of the cellular environment controls malignant cell phenotype and proliferation. However, the effect of viscous dissipation on these parameters has not yet been investigated, in part due to the lack of in vitro cell substrates reproducing the mechanical properties of normal tissues and tumors. In this article, we use a newly reported viscoelastic polyacrylamide gel cell substrate, and we characterize the impact of viscous dissipation on three malignant cell lines: DU145 and PC3 derived from prostate and LN229 from brain. The spreading, motility and proliferation rates of these cells were analyzed on 1 kPa and 5 kPa elastic and viscoelastic gels. Surprisingly, the effect of substrate viscous dissipation on cell behavior depended on substrate stiffness for the three cell types tested. We conclude that viscoelasticity controls the spreading, proliferation and migration of malignant cells in vitro. These results highlight the critical role of viscous dissipation in the phenotype and proliferation of malignant cells, especially in stiff tumor environments. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7892690&blobtype=pdf |
| ISSN | 16177959 |
| Journal | Biomechanics and Modeling in Mechanobiology [Biomech Model Mechanobiol] |
| Volume Number | 20 |
| DOI | 10.1007/s10237-020-01374-9 |
| PubMed Central reference number | PMC7892690 |
| Issue Number | 1 |
| PubMed reference number | 32785801 |
| e-ISSN | 16177940 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2020-08-12 |
| Publisher Place | Berlin/Heidelberg |
| Access Restriction | Open |
| Rights License | Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. © The Author(s) 2020 |
| Subject Keyword | Viscoelasticity Malignant cell Mechanosensing Viscosity sensing |
| Content Type | Text |
| Resource Type | Article |
| Subject | Mechanical Engineering Modeling and Simulation Biotechnology |
