Human primed ILCPs support endothelial activation through NF-κB signaling.

Content Provider	Europe PMC
Author	Vanoni, Giulia Ercolano, Giuseppe Candiani, Simona Rutigliani, Mariangela Lanata, Mariangela Derré, Laurent Marcenaro, Emanuela Schneider, Pascal Romero, Pedro Jandus, Camilla Trabanelli, Sara
Editor	Cerullo, Vincenzo Taniguchi, Tadatsugu
Copyright Year	2021
Abstract	Innate lymphoid cells (ILCs) represent the most recently identified subset of effector lymphocytes, with key roles in the orchestration of early immune responses. Despite their established involvement in the pathogenesis of many inflammatory disorders, the role of ILCs in cancer remains poorly defined. Here we assessed whether human ILCs can actively interact with the endothelium to promote tumor growth control, favoring immune cell adhesion. We show that, among all ILC subsets, ILCPs elicited the strongest upregulation of adhesion molecules in endothelial cells (ECs) in vitro, mainly in a contact-dependent manner through the tumor necrosis factor receptor- and RANK-dependent engagement of the NF-κB pathway. Moreover, the ILCP-mediated activation of the ECs resulted to be functional by fostering the adhesion of other innate and adaptive immune cells. Interestingly, pre-exposure of ILCPs to human tumor cell lines strongly impaired this capacity. Hence, the ILCP–EC interaction might represent an attractive target to regulate the immune cell trafficking to tumor sites and, therefore, the establishment of an anti-tumor immune response.
Journal	eLife
Volume Number	10
PubMed Central reference number	PMC7891932
PubMed reference number	33554861
e-ISSN	2050084X
DOI	10.7554/elife.58838
Language	English
Publisher	eLife Sciences Publications, Ltd
Publisher Date	2021-02-08
Access Restriction	Open
Rights License	This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. © 2021, Vanoni et al
Subject Keyword	endothelium innate lymphoid cells NF-κB Human
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Neuroscience Medicine Biochemistry, Genetics and Molecular Biology