MicroRNA‑149‑3p inhibits cell proliferation by targeting AKT2 in oral squamous cell carcinoma.

Content Provider	Europe PMC
Author	Shen, Qin Zhu, Hong Lei, Qiaoling Chen, Luyuan Yang, Dajiang Sui, Wen
Copyright Year	2020
Abstract	MicroRNAs (miRs) exhibit oncogenic or tumor suppressive functions that contribute to the initiation and development of various types of human cancer. miR-149-3p has been reported to serve multiple roles in the regulation of proliferation, apoptosis and metastasis. However, the effects and detailed mechanism of miR-149-3p in oral squamous cell carcinoma (OSCC) remain unclear. In the present study, miR-149-3p mimic, mimic control, miR-149-3p inhibitor and inhibitor control were transiently transfected into Cal27 and SCC-9 cells. The viability, proliferation and apoptosis of OSCC cells were determined using Cell Counting Kit-8, colony formation and Annexin V assays, respectively. The mRNA expression levels of miR-149-3p and AKT2 were determined by reverse transcription-quantitative PCR. The protein expression levels of AKT2, cleaved caspase-3 and cleaved PARP were examined by western blot analysis. The binding of miR-149-3p to the AKT2 3′-untranslated region was evaluated by a dual luciferase reporter assay. In the present study, overexpression of miR-149-3p reduced the viability and proliferation of OSCC cells. By contrast, increased cell viability and proliferation was observed in miR-149-3p-deficient OSCC cells. Dual luciferase reporter assay indicated that miR-149-3p significantly decreased the luciferase activity of the wild-type AKT2 3′-untranslated region. Moreover, overexpression of miR-149-3p downregulated the mRNA and protein expression levels of AKT2, suggesting that miR-149-3p was a negative modulator of AKT2. Restoration of AKT2 efficiently reversed the miR-149-3p-mediated reduction in the proliferative capacity of OSCC cells. In addition, miR-149-3p enhanced the sensitivity of OSCC cells to the chemotherapeutic drug 5-fluorouracil. Taken together, the current findings revealed an inhibitory effect of miR-149-3p on the proliferation of OSCC cells through the post-transcriptional suppression of AKT2, and indicated a potential chemosensitizing function of miR-149-3p for the treatment of patients with OSCC.
ISSN	17912997
Journal	Molecular Medicine Reports
Volume Number	23
PubMed Central reference number	PMC7821286
Issue Number	3
PubMed reference number	33398370
e-ISSN	17913004
DOI	10.3892/mmr.2020.11811
Language	English
Publisher	D.A. Spandidos
Publisher Date	2021-01-05
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Shen et al.
Subject Keyword	oral squamous cell carcinoma microRNA-149-3p AKT2 cell proliferation
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Biochemistry Cancer Research Molecular Medicine Oncology