Genome Sequence, Proteome Profile, and Identification of a Multiprotein Reductive Dehalogenase Complex in Dehalogenimonas alkenigignens Strain BRE15M.

Content Provider	Europe PMC
Author	Trueba-Santiso, Alba Wasmund, Kenneth Soder-Walz, Jesica M. Marco-Urrea, Ernest Adrian, Lorenz
Copyright Year	2020
Abstract	Bacteriaof the genus Dehalogenimonas respirewith vicinally halogenated alkanes via dihaloelimination.We aimed to describe involved proteins and their supermolecular organization.Metagenomic sequencing of a Dehalogenimonas-containing culture resulted in a 1.65 Mbp draft genome of Dehalogenimonas alkenigignens strain BRE15M. It contained31 full-length reductive dehalogenase homologous genes (rdhA), but only eight had cognate rdhB gene coding formembrane-anchoring proteins. Shotgun proteomics of cells grown with1,2-dichloropropane as an electron acceptor identified 1152 proteinsrepresenting more than 60% of the total proteome. Ten RdhA proteinswere detected, including a DcpA ortholog, which was the strongestexpressed RdhA. Blue native gel electrophoresis(BNE) demonstrating maximum activity was localized in a protein complexof 146–242 kDa. Protein mass spectrometry revealed the presenceof DcpA, its membrane-anchoring protein DcpB, two hydrogen uptakehydrogenase subunits (HupL and HupS), an iron–sulfur protein(HupX), and subunits of a redox protein with a molybdopterin-bindingmotif (OmeA and OmeB) in the complex. BNE after protein solubilizationwith different detergent concentrations revealed no evidence for aninteraction between the putative respiratory electron input module(HupLS) and the OmeA/OmeB/HupX module. All detected RdhAs comigratedwith the organohalide respiration complex. Based on genomic and proteomicanalysis, we propose quinone-independent respiration in Dehalogenimonas.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7786376&blobtype=pdf
ISSN	15353893
Journal	Journal of Proteome Research [J Proteome Res]
Volume Number	20
DOI	10.1021/acs.jproteome.0c00569
PubMed Central reference number	PMC7786376
Issue Number	1
PubMed reference number	32975419
e-ISSN	15353907
Language	English
Publisher	American ChemicalSociety
Publisher Date	2020-10-07
Access Restriction	Open
Rights License	This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited. © 2020 AmericanChemical Society
Subject Keyword	organohalide respiration dihaloelimination Dehalogenimonas alkenigignens strain BRE15M Dehalococcoidia genome sequencing 1,2-dichloropropane proteome profiling
Content Type	Text
Resource Type	Article
Subject	Chemistry Biochemistry