NT-3 promotes osteogenic differentiation of mouse bone marrow mesenchymal stem cells by regulating the Akt pathway.

Content Provider	Europe PMC
Author	Zhang, Shanqiang Sun, Shizhu He, Jun Shen, Lei
Copyright Year	2020
Abstract	Objectives:To investigate the effect of neurotrophin-3 (NT-3) on osteogenic/adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs).Methods:Osteogenic differentiation was detected by alkaline phosphatase (ALP) staining and alizarin red staining (ARS). Adipogenic differentiation was detected by oil red O (ORO) staining. The expression of bone-related genes (Runx2, Osterix, OCN, ALP) and lipogenic genes (FABP4, PPAR, CEBP, LPL) was detected by real-time quantitative polymerase chain reaction (real-time qPCR). The expression of p-Akt and Akt protein was detected by Western blot assay.Results:ALP staining and ARS staining showed that the overexpression of NT-3 could promote the differentiation into osteoblasts, while knockdown of NT-3 could inhibit that. Real-time qPCR showed that the overexpression of NT-3 could increase the expression of osteoblast genes, while knockdown of NT-3 could inhibit that. ORO staining showed that the overexpression of NT-3 could inhibit the differentiation into adipogenesis, while knockdown of NT-3 can promote that. Real-time qPCR showed that the overexpression of NT-3 could reduce the expression of lipogenic genes. while knockdown NT-3 could increase that. In addition, the overexpression of NT-3 increased p-Akt/Akt levels significantly, while knockdown NT-3 reduced that significantly.Conclusion:NT-3 could promote the differentiation of mouse BMSCs into osteoblasts and inhibit their differentiation into adipogenesis.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7716693&blobtype=pdf
ISSN	11087161
Journal	Journal of Musculoskeletal & Neuronal Interactions [J Musculoskelet Neuronal Interact]
Volume Number	20
PubMed Central reference number	PMC7716693
Issue Number	4
PubMed reference number	33265088
Language	English
Publisher	International Society of Musculoskeletal and Neuronal Interactions
Publisher Date	2020-12-01
Publisher Place	Greece
Access Restriction	Open
Rights License	This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 4.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright: © Journal of Musculoskeletal and Neuronal Interactions
Subject Keyword	Adipogenic differentiation Bone marrow mesenchymal stem cells (BMSCs) Neurotrophin-3 (NT-3) Osteogenic differentiation
Content Type	Text
Resource Type	Article
Subject	Orthopedics and Sports Medicine Physiology Endocrinology, Diabetes and Metabolism