NDLI: Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in <i>Cornu Saigae Tataricae</i>.

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Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae.

Content Provider	Europe PMC
Author	Chen, Yufei Yang, Yaya Qian, Yinhuan Gblinwon, Roselyn Tehzee Jiao, Zhaoqun Chen, Liqun Lin, Ling Zheng, Yang Yang, Huan Shen, Yuping
Copyright Year	2019
Abstract	AbstractA multiplex PCR assay was developed to simultaneously differentiate four antelope species and identify adulteration in Cornu Saigae Tataricae. Four novel primer sets were designed with high inter-species specificity and intra-species stability. Limit of detection was estimated to be 10 ng of genomes. When a mixture of antelope hornand fake species was assayed, it exhibited powerful differentiation capability. 5 out of 12 batches of commercialproducts were identified to be counterfeited or adulterated with Ovis aries Linnaeus and/or Capra hircus Linnaeus. It is readily applicable in routine analysis for identification of sham or adulterants of Cornu Saigae Tataricae.
Page Count	5
Volume Number	4
PubMed Central reference number	PMC7707849
Issue Number	2
PubMed reference number	33365929
Journal	Mitochondrial DNA. Part B, Resources [Mitochondrial DNA B Resour]
e-ISSN	23802359
DOI	10.1080/23802359.2019.1666667
Language	English
Publisher	Taylor & Francis
Publisher Date	2019-09-25
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Subject Keyword	Mitochondrial DNA species-specific primer multiplex PCR assay antelope horn animal-derived Chinese medicine species identification
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology

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Harnessing multiplex PCR assay targeting specific mitochondrial DNA elements for simultaneous identification of antelope species in Cornu Saigae Tataricae.

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