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Activation of a [NiFe]-hydrogenase-4 isoenzyme by maturation proteases.
| Content Provider | Europe PMC |
|---|---|
| Author | Finney, Alexander J. Buchanan, Grant Palmer, Tracy Coulthurst, Sarah J. Sargent, Frank |
| Copyright Year | 2020 |
| Abstract | Maturation of [NiFe]-hydrogenases often involves specific proteases responsible for cleavage of the catalytic subunits. Escherichia coli HycI is the protease dedicated to maturation of the Hydrogenase-3 isoenzyme, a component of formate hydrogenlyase-1. In this work, it is demonstrated that a Pectobacterium atrosepticum HycI homologue, HyfK, is required for hydrogenase-4 activity, a component of formate hydrogenlyase-2, in that bacterium. The P. atrosepticum ΔhyfK mutant phenotype could be rescued by either P. atrosepticum hyfK or E. coli hycI on a plasmid. Conversely, an E. coli ΔhycI mutant was complemented by either E. coli hycI or P. atrosepticum hyfK in trans. E. coli is a rare example of a bacterium containing both hydrogenase-3 and hydrogenase-4, however the operon encoding hydrogenase-4 has no maturation protease gene. This work suggests HycI should be sufficient for maturation of both E. coli formate hydrogenlyases, however no formate hydrogenlyase-2 activity was detected in any E. coli strains tested here. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7654741&blobtype=pdf |
| ISSN | 13500872 |
| Journal | Microbiology [Microbiology (Reading)] |
| Volume Number | 166 |
| DOI | 10.1099/mic.0.000963 |
| PubMed Central reference number | PMC7654741 |
| Issue Number | 9 |
| PubMed reference number | 32731905 |
| e-ISSN | 14652080 |
| Language | English |
| Publisher | Microbiology Society |
| Publisher Date | 2020-09-01 |
| Access Restriction | Open |
| Rights License | This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution. © 2020 The Authors |
| Subject Keyword | Escherichia coli Pectobacterium atrosepticum formate hydrogenlyase hydrogenase maturase protease |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology |
