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Expression profiles of the Wnt/β-catenin signaling-related extracellular antagonists during proliferation and differentiation in human osteoblast-like cells.
| Content Provider | Europe PMC |
|---|---|
| Author | Parra-Torres, Alma Y. Enríquez, Juana Jiménez-Ortega, Rogelio F. Patiño, Nelly Castillejos-López, Manuel De Jesús Torres-Espíndola, Luz M. Ramírez-Salazar, Eric G. Velázquez-Cruz, Rafael |
| Copyright Year | 2020 |
| Abstract | Bone formation is a dynamic process directed by osteoblast activity. The transition from the proliferation to differentiation stage during osteoblast maturation involves the downregulation of the Wnt/β-catenin signaling pathway, and extracellular antagonists are important for the regulation of Wnt signaling. However, the expression levels of Wnt antagonists in these stages of human osteoblast maturation have not been fully elucidated. Therefore, the aim of the present study was to investigate the expression levels of extracellular Wnt antagonists during proliferation and differentiation in osteoblast-like cell lines. The results demonstrated an overlap between the differential expression of secreted Frizzled-related protein (SFPR)2, SFRP3, SFRP4 and Dickkopf (DKK) 2 genes during the differentiation stage in the MG-63 and Saos-2 cells. Furthermore, high expression levels of DKK3 in MG-63 cells, Wnt inhibitory factor 1 (WIF1) in Saos-2 cells and DKK4 in hFOB 1.19 cells during the same stage (differentiation), were observed. The upregulated expression levels of Wnt antagonists were also correlated with the high expression of anxin 2 during the differentiation stage. These findings suggested that Wnt-related antagonists could modulate the Wnt/β-catenin signaling pathway. By contrast, DKK1 was the only gene that was found to be upregulated during the proliferation stage in hFOB 1.19 and Saos-2 cells. To the best of our knowledge, the present study provides, for the first time, the expression profile of Wnt antagonists during the proliferation stage and the initial phases of differentiation in osteoblast-like cell lines. The current results offer a basis to investigate potential targets for bone-related Wnt-signaling modulation in bone metabolism research. |
| ISSN | 17920981 |
| Journal | Experimental and Therapeutic Medicine |
| Volume Number | 20 |
| PubMed Central reference number | PMC7654218 |
| Issue Number | 6 |
| PubMed reference number | 33178352 |
| e-ISSN | 17921015 |
| DOI | 10.3892/etm.2020.9384 |
| Language | English |
| Publisher | D.A. Spandidos |
| Publisher Date | 2020-10-23 |
| Access Restriction | Open |
| Rights License | This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Parra-Torres et al. |
| Subject Keyword | osteoblasts Wnt/β-catenin pathway extracellular Wnt antagonists osteoblast differentiation osteoblast proliferation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Microbiology Cancer Research |
