Quantitative Proteomic Analysis of Porcine Intestinal Epithelial Cells Infected with Porcine Deltacoronavirus Using iTRAQ-Coupled LC-MS/MS.

Content Provider	Europe PMC
Author	Zhou, Xinrong Zhou, Lei Ge, Xinna Guo, Xin Han, Jun Zhang, Yongning Yang, Hanchun
Copyright Year	2020
Abstract	Porcinedeltacoronavirus (PDCoV) is an emergent enteropathogeniccoronavirus associated with swine diarrhea. Porcine small intestinalepithelial cells (IPEC) are the primary target cells of PDCoV infectionin vivo. Here, isobaric tags for relative and absolute quantification(iTRAQ) labeling coupled to liquid chromatography–tandem massspectrometry (LC-MS/MS) was used to quantitatively identify differentiallyexpressed proteins (DEPs) in PDCoV-infected IPEC-J2 cells. A totalof 78 DEPs, including 23 upregulated and 55 downregulated proteins,were identified at 24 h postinfection. The data are available viaProteomeXchange with identifier PXD019975. To ensure reliability ofthe proteomics data, two randomly selected DEPs, the downregulatedanaphase-promoting complex subunit 7 (ANAPC7) and upregulated interferon-inducedprotein with tetratricopeptide repeats 1 (IFIT1), were verified byreal-time PCR and Western blot, and the results of which indicatethat the proteomics data were reliable and valid. Bioinformatics analyses,including GO, COG, KEGG, and STRING, further demonstrated that a majorityof the DEPs are involved in numerous crucial biological processesand signaling pathways, such as immune system, digestive system, signaltransduction, RIG-I-like receptor, mTOR, PI3K-AKT, autophagy, andcell cycle signaling pathways. Altogether, this is the first studyon proteomes of PDCoV-infected host cells, which shall provide valuableclues for further investigation of PDCoV pathogenesis.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7640975&blobtype=pdf
ISSN	15353893
Journal	Journal of Proteome Research [J Proteome Res]
Volume Number	19
DOI	10.1021/acs.jproteome.0c00592
PubMed Central reference number	PMC7640975
Issue Number	11
PubMed reference number	33045833
e-ISSN	15353907
Language	English
Publisher	American ChemicalSociety
Publisher Date	2020-10-12
Access Restriction	Open
Rights License	This article is made available via the PMC Open Access Subset for unrestricted RESEARCH re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. © 2020 AmericanChemical Society
Subject Keyword	porcine deltacoronavirus (PDCoV) IPEC-J2cells isobaric tags for relative and absolute quantification(iTRAQ) liquid chromatography−tandem mass spectrometry(LC-MS/MS) proteome
Content Type	Text
Resource Type	Article
Subject	Chemistry Biochemistry