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Evaluation of Bone Marrow-derived Stem Cells and Adipose-derived Stem Cells Co-cultured on Human Nucleus Pulposus Cells: A Pilot Study.
| Content Provider | Europe PMC |
|---|---|
| Author | Yoon, Sang Hoon Kim, Dae Hee Cho, Sam Kim, Ki-Jeong |
| Copyright Year | 2020 |
| Abstract | ObjectiveWe aimed to determine whether bone marrow-derived mesenchymal stem cells (BDMSCs) effectively attenuate the degeneration of human nucleus pulposus cells (NPCs).MethodsFour NPC lines were obtained from 3 subjects who underwent spinal surgery for cervical disc herniation (n=1) or lumbar disc herniation (n=2). For co-culture wells without contact, BDMSCs and adipose-derived mesenchymal stem cells (ADMSCs) were seeded on tissue culture plates and maintained for 3 days. Senescence-associated β-gal (SA-β-gal) staining was represented as a percentage of the total number of stained cells (%). The cells with intracellular lipid droplets (LDs) were represented as the percentage of the number of cells with LDs. Glycosaminoglycan (GAG) secretion was measured at 450 nm, using a commercial kit, to analyze optical density.ResultsThe ratio of cells stained with SA-β-gal to the total number of cells reduced significantly when co-cultured with BDMSCs and ADMSCs (p<0.001 vs. p<0.001). The proportion of NPCs containing LDs was lower when co-cultured with BDMSCs than with ADMSCs (p<0.001). The optical density related to GAG secretion was lower in BDMSCs and ADMSCs co-cultured with NPCs than in the controls (p<0.001 vs. p<0.001).ConclusionSA-β-gal staining showed significant attenuation of degenerative changes in NPCs co-cultured with BDMSCs. Moreover, the unexpected increase in LDs was significantly higher in NPCs co-cultured with ADMSCs than in those co-cultured with BDMSCs. However, GAG secretion was significantly decreased in NPCs co-cultured with MSCs. |
| Related Links | https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7607015&blobtype=pdf |
| ISSN | 22348999 |
| Journal | Korean Journal of Neurotrauma [Korean J Neurotrauma] |
| Volume Number | 16 |
| DOI | 10.13004/kjnt.2020.16.e36 |
| PubMed Central reference number | PMC7607015 |
| Issue Number | 2 |
| PubMed reference number | 33163421 |
| e-ISSN | 22882243 |
| Language | English |
| Publisher | Korean Neurotraumatology Society |
| Publisher Date | 2020-10-16 |
| Access Restriction | Open |
| Rights License | This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Copyright © 2020 Korean Neurotraumatology Society |
| Subject Keyword | Bone marrow mesenchymal stem cells Adipose-derived mesenchymal stem cells Nucleus pulposus cell Co-culture |
| Content Type | Text |
| Resource Type | Article |
| Subject | Surgery |
