Evaluation of Better Staining Method among Hematoxylin and Eosin, Giemsa and Periodic Acid Schiff-Alcian Blue for the Detection of Helicobacter pylori in Gastric Biopsies.

Content Provider	Europe PMC
Author	Alkhamiss, Abdullah Saleh
Copyright Year	2020
Abstract	BackgroundThis study was undertaken to evaluate the preferred method (Giemsa or periodic acid Schiff-Alcian blue [PAS-AB] stains) of detecting Helicobacter pylori (H. pylori) in gastric mucosal biopsies in terms of sensitivity, specificity and applicability. To the best of my knowledge, this is the first report comparing Giemsa and PAS-AB staining for the detection of H. pylori in such biopsies.MethodsThe formalin-fixed paraffin-embedded blocks of 49 gastric biopsies from different patients were collected from the archive of anatomical pathology at King Abdulaziz Medical City, National Guard, Riyadh, Saudi Arabia. From each block, three slides were prepared and analysed using the hematoxylin and eosin (H&E), Giemsa and PAS-AB stains to detect the presence/absence of H. pylori, and the results were compared in terms of sensitivity, specificity and applicability.ResultsThe majority of the biopsies in this study showed antrum-type gastric mucosa. Only 15 biopsies showed active gastritis, whereas the rest showed chronic gastritis. Three biopsies showed intestinal metaplasia. All were detected by PAS-AB stain, but only two-thirds were detected by H&E stain. Fifteen gastric biopsies showed H. pylori infection in general and in 13 of them, active gastritis cases were discovered. Fourteen out of these 15 H. pylori infection cases were detected by Giemsa stain, whereas only 13 cases were detected by H&E stain. PAS-AB stain showed the worst results since it demonstrated only 40% sensitivity and 67.65% specificity in H. pylori detection.ConclusionGiemsa stain has better sensitivity and specificity in gastric H. pylori infection detection than PAS-AB. Therefore, using PAS-AB stain to detect H. pylori infection is not recommended.
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ISSN	1394195X
Journal	The Malaysian Journal of Medical Sciences : MJMS [Malays J Med Sci]
Volume Number	27
DOI	10.21315/mjms2020.27.5.6
PubMed Central reference number	PMC7605829
Issue Number	5
PubMed reference number	33154702
e-ISSN	21804303
Language	English
Publisher	Penerbit Universiti Sains Malaysia
Publisher Date	2020-10-27
Access Restriction	Open
Rights License	This work is licensed under the terms of the Creative Commons Attribution (CC BY) (http://creativecommons.org/licenses/by/4.0/). © Penerbit Universiti Sains Malaysia, 2020
Subject Keyword	gastric biopsies gastritis Helicobacter pylori intestinal metaplasia Giemsa periodic acid Schiff-Alcian blue
Content Type	Text
Resource Type	Article
Subject	Medicine