Anti-inflammatory Bifidobacterium strains prevent dextran sodium sulfate induced colitis and associated gut microbial dysbiosis in mice.

Content Provider	Europe PMC
Author	Singh, Shashank Bhatia, Ruchika Khare, Pragyanshu Sharma, Shikha Rajarammohan, Sivasubramanian Bishnoi, Mahendra Bhadada, Sanjay Kumar Sharma, Shyam Sunder Kaur, Jaspreet Kondepudi, Kanthi Kiran
Abstract	Crohn's and ulcerative colitis are common inflammatory conditions associated with Inflammatory bowel disease. Owing to the importance of diet based approaches for the prevention of inflammatory gut conditions, the present study was aimed to screen the human isolates of Bifidobacterium strains based on their ability to reduce LPS-induced inflammation in murine macrophage (RAW 264.7) cells and to evaluate prioritized strains for their preventive efficacy against ulcerative colitis in mice. Twelve out of 25 isolated strains reduced the production of LPS-induced nitric oxide and inflammatory cytokines. Furthermore, three strains, B. longum Bif10, B. breve Bif11, and B. longum Bif16 conferred protection against dextran sodium sulfate induced colitis in mice. The three strains prevented shortening of colon, spleen weight, percentage body weight change and disease activity index relative to colitis mice. Lower levels of Lipocalin-2, TNF-α, IL-1β and IL-6 and improved SCFA levels were observed in Bifidobacterium supplemented mice relative to DSS counterparts. Bacterial composition of B. longum Bif10 and B. breve Bif11 fed mice was partly similar to the normal mice, while DSS and B. longum Bif16 supplemented mice showed deleterious alterations. At the genus level, Bifidobacterium supplementation inhibited the abundances of pathobionts such as Haemophilus, Klebsiella and Lachnospira there by conferring protection.
Journal	Scientific Reports [Sci Rep]
Volume Number	10
DOI	10.1038/s41598-020-75702-5
PubMed Central reference number	PMC7596498
Issue Number	1
PubMed reference number	33122795
e-ISSN	20452322
Language	English
Publisher	Nature Publishing Group
Publisher Date	2020-10-29
Publisher Place	London
Access Restriction	Open
Subject Keyword	Dysbiosis Ulcerative colitis
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary