Circular RNA hsa_circ_0003204 promotes cervical cancer cell proliferation, migration, and invasion by regulating MAPK pathway.

Content Provider	Europe PMC
Author	Huang, Xiao-Bin Song, Kai-Jing Chen, Guo-Bin Liu, Rui Jiang, Zhuo-Fei He, Yuan-Li
Copyright Year	2020
Abstract	ABSTRACTCervical cancer (CC) is the second most common malignancy in women worldwide. The mechanism underlying CC development remains unclear. Recently, Circular RNAs (circRNAs)have attracted attention because of its role in tumorigenesis. To investigate circRNAsin CC, RNA sequencing was employed to characterize circRNA expression profile between CC tissues and matched adjacent normal tissues. The expression of hsa_circ_0003204 was examined in CC tissues and cell lines by real-time PCR. Migration assay and invasion assay were used to verify the effect of hsa_circ_0003204 on migration and invasion ability in CC cell lines. Tumor formation assay in nude mice was used to analyze the effect of hsa_circ_0003204 on the tumorigenicity of CC cell lines in vitro. Western blotting analyzes were performed to investigate the role of hsa_circ_0003204 in the regulation of MAPK signaling activation. We found that circRNA hsa_circ_0003204 was significantly upregulated in CC tissues. The function and potential molecular mechanisms of hsa_circ_0003204 were also investigated in vitro and in vivo. Hsa_circ_0003204 knockdown reduced cell growth, migration, and invasion but promoted cells apoptosis. However, the over-expression of hsa_circ_0003204 had the opposite effect. The MAPK pathway was different in hsa_circ_0003204 over-expression or down-expression cells, compared to parental cells. In addition, over-expression of hsa_circ_0003204 significantly increased tumor volume and tumor weight in vivo.Taken together, results indicated hsa_circ_0003204 may serve as a potential therapeutic target for patients with CC.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7583705&blobtype=pdf
Page Count	11
ISSN	15384047
Journal	Cancer Biology & Therapy [Cancer Biol Ther]
Volume Number	21
DOI	10.1080/15384047.2020.1824513
PubMed Central reference number	PMC7583705
Issue Number	10
PubMed reference number	33047994
e-ISSN	15558576
Language	English
Publisher	Taylor & Francis
Publisher Date	2020-10-13
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way. © 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.
Subject Keyword	circRNA cervical cancer cell proliferation cell apoptosis cell invasion and migration
Content Type	Text
Resource Type	Article
Subject	Cancer Research Molecular Medicine Pharmacology Oncology