Oral dysbiosis induced by Porphyromonas gingivalis is strain-dependent in mice.

Content Provider	Europe PMC
Author	Boyer, Emile Leroyer, Patricia Malherbe, Ludivine Fong, Shao Bing Loréal, Olivier Bonnaure Mallet, Martine Meuric, Vincent
Copyright Year	2020
Abstract	ABSTRACTBackground: Porphyromonas gingivalis strain W83, one of the most widely investigated, is considered virulent in the context of periodontitis. The recently isolated P. gingivalis TDC60 has been reported to be highly pathogenic, although it has not yet been investigated in a mouse periodontitis model by oral gavage.Aim: Our aim was to compare the virulence of both strains by evaluating their impact on alveolar bone loss and the composition of oral microbiota.Methods: We inoculated by oral gavage C57BL/6 mice with either one of the two P. gingivalis strains and compared to a sham-treated group, without antibiotics pre-treatment. The mandibular alveolar bone of treated mice and controls were assessed, one month after the final inoculation, by microCT measurements. Moreover, at this time, we characterized their oral microbiota by 16S rRNA gene sequencing.Results: While P. gingivalis W83 successfully initiated periodontitis, TDC60-treated mice only experienced moderate lesions. Furthermore, only W83-treated mice exhibited a specific distinct microbiota, with significantly lower richness and evenness than other samples, and decreased proportions of taxa usually found in healthy individuals.Conclusion: This association between alveolar bone loss and a major persistent shift of the oral microbiota gives insights into virulence discrepancies among these bacterial strains.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7580739&blobtype=pdf
Page Count	1
Journal	Journal of Oral Microbiology [J Oral Microbiol]
Volume Number	12
DOI	10.1080/20002297.2020.1832837
PubMed Central reference number	PMC7580739
Issue Number	1
PubMed reference number	33133418
e-ISSN	20002297
Language	English
Publisher	Taylor & Francis
Publisher Date	2020-10-12
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
Subject Keyword	Periodontitis animal model mice Porphyromonas gingivalis W83 P. gingivalis TDC60 oral microbiota alveolar bone loss
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology (medical) Dentistry