Development of an electrochemical biosensor for impedimetric detection of tetracycline in milk.

Content Provider	Europe PMC
Author	Mohammad-Razdari, Ayat Ghasemi-Varnamkhasti, Mahdi Rostami, Sajad Izadi, Zahra Ensafi, Ali A. Siadat, Maryam
Abstract	Abstract This study dealt with the fabrication of an impedimetric biosensor based on nanomaterial modified with pencil graphite electrode for the detection of tetracycline (TET) in milk samples. For response of the impedimetric aptasensor to be improved, the influence of different parameters (immobilization time of reduced grapheme oxide, time of aptamer, and TET binding, and concentration of aptamer) was optimized. In optimum conditions, the aptasensor provided a concentration range within 1 × 10–16 − 1 × 10–6 M and with a limit of detection of 3 × 10–17 M TET. The proposed impedimetric aptasensor was then used in milk samples analysis, and the acceptable recovery was achieved ranging from 92.8 to 102.1%. According to this study, the combination of an aptamer and electrochemical impedance spectroscopy is a promising method for detection of TET in milk samples with high reproducibility and stability. Graphic abstract
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7550490&blobtype=pdf
ISSN	00221155
Volume Number	57
DOI	10.1007/s13197-020-04506-2
PubMed Central reference number	PMC7550490
Issue Number	12
PubMed reference number	33087980
Journal	Journal of Food Science and Technology [J Food Sci Technol]
e-ISSN	09758402
Language	English
Publisher	Springer India
Publisher Date	2020-05-09
Publisher Place	New Delhi
Access Restriction	Open
Rights License	© Association of Food Scientists & Technologists (India) 2020
Subject Keyword	Aptasensor Antibiotics detection Nanocomposite Electrochemical impedance spectroscopy Reduced graphene oxide
Content Type	Text
Resource Type	Article
Subject	Food Science