Analysis of biomarkers and metabolic pathways in patients with unstable angina based on ultra‑high‑performance liquid chromatography‑quadrupole time‑of‑flight mass spectrometry.

Content Provider	Europe PMC
Author	Liu, Yuechen Li, Yue Zhang, Tianpu Zhao, Huan Fan, Simiao Cai, Xuemeng Liu, Yijia Li, Zhu Gao, Shan Li, Yubo Yu, Chunquan
Copyright Year	2020
Abstract	Unstable angina (UA) is a coronary disease with a high mortality and morbidity worldwide. The present study aimed to use non-invasive techniques to identify urine biomarkers in patients with UA, so as to provide more information for the early diagnosis and treatment of the disease. Based on metabolomics, urine samples from 28 patients with UA and 28 healthy controls (HCs) were analyzed using ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). A total of 16 significant biomarkers that could distinguish between patients with UA and HCs, including D-glucuronic acid, creatinine, succinic acid and N-acetylneuraminic acid, were identified. The major metabolic pathways associated with UA were subsequently analyzed by non-targeted metabolomics. The results demonstrated that amino acid and energy metabolism, fatty acid metabolism, purine metabolism and steroid hormone biosynthetic metabolism may serve important roles in UA. The results of the current study may provide a theoretical basis for the early diagnosis of UA and novel treatment strategies for clinicians. The trial was registered with the Chinese Clinical Trial Registration Center (registration no. ChiCTR-ROC-17013957) at Tianjin University of Traditional Chinese Medicine.
ISSN	17912997
Journal	Molecular Medicine Reports
Volume Number	22
PubMed Central reference number	PMC7533448
Issue Number	5
PubMed reference number	32901869
e-ISSN	17913004
DOI	10.3892/mmr.2020.11476
Language	English
Publisher	D.A. Spandidos
Publisher Date	2020-09-02
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Liu et al.
Subject Keyword	unstable angina urine metabolomics ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry biomarkers
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Biochemistry Cancer Research Molecular Medicine Oncology