Development of a biosensor from aptamers for detection of the porcine reproductive and respiratory syndrome virus.

Content Provider	Europe PMC
Author	Kuitio, Chakpetch Rasri, Natchaya Kiriwan, Duangnapa Unajak, Sasimanas Choowongkomon, Kiattawee
Copyright Year	2020
Abstract	BackgroundRecently, the pork industry of Thailand faced an epidemic of highly virulent strains of porcine reproductive and respiratory syndrome virus (PRRSV), which spread throughout Southeast Asia, including the Lao People's Democratic Republic and Cambodia. Hence, the rapid and on-site screening of infected pigs on a farm is essential.ObjectivesTo develop the new aptamer as a biosensor for detection PRRSV which are rapid and on-site screening of infected pig.MethodsNew aptamers against PRSSV were identified using the combined techniques of capillary electrophoresis, colorimetric assay by gold nanoparticles, and quartz crystal microbalance (QCM).ResultsThirty-six candidate aptamers of the PRRSV were identified from the systematic evolution of ligands by exponential enrichment (SELEX) by capillary electrophoresis. Only 8 out of 36 aptamers could bind to the PRSSV, as shown in a colorimetric assay. Of the 8 aptamers tested, only the 1F aptamer could bind specifically to the PRSSV when presented with the classical swine fever virus and a pseudo rabies virus. The QCM was used to confirm the specificity and sensitivity of the 1F aptamer with a detection limit of 1.87 × 1010 particles.ConclusionsSELEX screening of the aptamer equipped with capillary electrophoresis potentially revealed promising candidates for detecting the PRRSV. The 1F aptamer exhibited the highest specificity and selectivity against the PRRSV. These findings suggest that 1F is a promising aptamer for further developing a novel PRRSV rapid detection kit.
ISSN	1229845X
Journal	Journal of Veterinary Science
Volume Number	21
PubMed Central reference number	PMC7533388
Issue Number	5
PubMed reference number	33016024
e-ISSN	1976555X
DOI	10.4142/jvs.2020.21.e79
Language	English
Publisher	The Korean Society of Veterinary Science
Publisher Date	2020-09-01
Access Restriction	Open
Rights License	This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. © 2020 The Korean Society of Veterinary Science
Subject Keyword	Porcine reproductive and respiratory syndrome virus detection aptamer l biosensor gold nanoparticles QCM
Content Type	Text
Resource Type	Article
Subject	Veterinary